孕晚期小鼠巨噬细胞抗原递呈功能对Th1/Th2免疫的影响

目的 探讨孕晚期Balb/c小鼠脾脏巨噬细胞抗原提呈功能与母体Th2＞Th1免疫偏倚的关系.方法 选取孕晚期Balb/c小鼠作为研究对象,以动情期小鼠为对照.将Balb/c小鼠脾脏巨噬细胞经抗原激发后作为抗原递呈细胞(APC),一部分(1°APC)用于致敏小鼠T细胞,然后分离致敏T细胞,再将另一部分巨噬细胞(2°APC)与致敏T细胞混合培养.采用淋巴细胞抗原特异性转化实验检验孕晚期小鼠脾脏巨噬细胞抗原提呈功能的改变,采用流式细胞技术检测CD4、CD8、IL-10和IFN-γ在反应后T细胞上的表达. 结果以孕晚期Balb/c小鼠脾脏巨噬细胞作为1°APC致敏动情期小鼠,再以孕晚期Balb/c小鼠脾脏巨噬细胞作为2°APC体外诱导致敏T细胞增殖的强度与对照组作为2°APC比较,差异无统计学意义(P＞0.05);以动情期Balb/c小鼠脾脏巨噬细胞作为1°APC,再以孕晚期Balb/c小鼠脾脏巨噬细胞作为2°APC诱导致敏T细胞增殖的强度则低于对照组作为2°APC时的增殖强度(P＜0.05).孕晚期小鼠脾脏巨噬细胞作为2°APC可诱导更多IL-10 T细胞产生(P＜0.05);IFN-γ T细胞的比例与1°或2°APC的类型无关. 结论孕晚期Balb/c小鼠脾脏巨噬细胞不是一种有效的APC细胞,可诱导孕期母体的Th2型免疫,并使Th1型免疫维持一定水平,对正常妊娠具有重要意义.

The Affection of Antigen Presentation Ability, which Sources from the Spleen Macrophage of Balb/c Mouse during Late Gestation, on Maternal Th2/Th1 Type of Immune

OBJECTIVE: To study the relationship between antigen presentation ability of spleen macrophage and maternal Th2>Th1 immune bias in Balb/c mice during late pregnancy. METHODS: Balb/c mice during late gestation were adopted in our study, and mice of same species in estrus were used as control. With antigen stimulation, the spleen macrophages of Balb/c mice were pulsed as antigen presentation cells (APC). T cells sensitized previously by pulsed macrophage (1 degree APC) were cultured in mixture with macrophage pulsed by same antigen (2 degrees APC). An antigen special lymphocyte transformation test in vitro was used to evaluate the antigen presentation ability of spleen macrophage from mice of late gestation, and a flow cytometry method was used to measured the ration of CD4, CD8, IL-10 and IFN-gamma positive cell in T cells which had being induced to proliferate. RESULTS: When spleen macrophage from mice during late gestation was used as 1 degree APC, the proliferation of sensitized T cell induced by macrophage from late pregnancy mice used as 2 degree APC was no more intense than that from estrous mice (P > 0.05). When spleen macrophage from mice in oestrus was used as 1 degree APC, the proliferation of sensitized T cell induced by macrophage from late pregnancy mice as 2 degrees APC was lower intense than that from estrous mice (P < 0.05). The type of 1 degree APC did not affect the ratio of IL-10 positive T cell, and macrophage from late pregnancy mice could induce more IL-10 positive T cell than that from estrous mice when they were used as 2 degrees APC (P < 0.05). The type of 1 degree or 2 degrees APC did not affect the ratio of IFN-gamma positive T cell. CONCLUSION: The spleen macrophage from mice during late gestation is not an effective APC, but can induce maternal Th2 type of immune and maintain the Th1 type immune at a lower stage during pregnancy, which means it may has some important role in pregnancy.