三磷酸腺苷二钠对慢性高原病大鼠肝脏保护作用

 为研究三磷酸腺苷二钠对慢性高原病(chronic mountain sickness,CMS)大鼠肝脏的保护作用,取60 只SD 大鼠,对其中50 例进行慢性高原病造模后,随机分为高原模型组、硝苯地平对照组、三磷酸腺苷二钠低剂量组、三磷酸腺苷二钠中剂量组、三磷酸腺苷二钠高剂量组,每组10 只;剩余10 只平原环境饲养为正常对照组;造模30 d 后,根据大鼠体重进行干预。连续灌胃15d 后,测定肺动脉压(pulmonary artery pressure,PAP)、同型半胱氨酸(homocysteine,Hcy)、白介素-6(interleukin-6,IL-6)、C-反应蛋白(C-reactionprotein,CRP)、超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-PX)、谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartatetransaminase,AST)等指标。结果发现,与正常对照组比较,高原模型组和硝苯地平对照组大鼠体内的Hcy,IL-6,CRP,MDA,ALT 和AST 水平升高,SOD 和GSH-PX 水平降低,PAP 升高,提示高原低氧对大鼠的肝脏造成损害。与高原模型组比较,三磷酸腺苷二钠高剂量组大鼠体内的Hcy,IL-6,CRP,MDA,ALT 和AST 水平降低(P < 0.05),SOD 和GSH-PX 水平升高(P < 0.05)。故而认为高剂量的三磷酸腺苷二钠对CMS 大鼠肝脏有保护作用。

Protective effect of trinosin on the liver of chronic mountain sickness rat model

To study the protective effect of trinosin on the liver of chronic mountain sickness (CMS) rat model, CMS was induced in 50 of 60 SD rats,which were randomly divided into CMS model group, nifedipine control group, high, medium, low dosage trinosin groups and blank control group (10 each). Fifty rats were in low pressure oxygen cabin for 30 days except for the blank control group. After continuous gavage administrationfor 15 days according to their weights,we tested the indexes of pulmonary artery pressure (PAP), homocysteine (Hcy), interleukin- 6 (IL- 6), C- reactionprotein (CRP), superoxide dismutase (SOD), malondialdehyde(MDA), glutathione peroxidase (GSH- PX), alanine aminotransferase (ALT), and aspartate transaminase (AST). The results showed that compared with the blank control group, the CMS model group and nifedipine control group had higher levels of PAP, Hcy, IL-6, CRP, MDA, ALT and AST (P < 0.05), but lower levels of SOD and GSH-PX (P < 0.05), indicating the rat liver was damaged under hypoxic condition. Compared with the CMS model group, the high dosage trinosin group had lower levels ofHcy, IL-6, CRP, MDA, ALT and AST (P < 0.05), but higher levels of SOD and GSH-PX (P < 0.05), indicatinghigh dosage of trinosin had protective effect on the liver of CMS rat model.