| Notch 3沉默对人急性T淋巴细胞白血病细胞增殖与凋亡的影响 |
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| 引用本文： | 项捷,欧阳永日,崔颖,林芳,任继鸿,龙敏,陈曦,卫军霞,张惠中. Notch 3沉默对人急性T淋巴细胞白血病细胞增殖与凋亡的影响[J]. 现代肿瘤医学, 2011, 19(8): 1482-1487. DOI: 10.3969/j.issn.1672-4992.2011.08.03 |
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| 作者姓名： | 项捷 欧阳永日 崔颖 林芳 任继鸿 龙敏 陈曦 卫军霞 张惠中 |
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| 作者单位： | 第四军医大学唐都医院中心实验室,陕西,西安,710038 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的:探讨Notch 3受体特异性沉默对急性T淋巴细胞白血病的影响。方法:运用survivin启动子介导的RNA干涉方法特异性地封闭急性T淋巴细胞白血病细胞SupT1中Notch 3基因的表达。结果:survivin启动子介导的RNA干涉系统能特异而高效封闭肿瘤细胞内源Notch 3基因的表达,Notch 3基因表达的下调能够使肿瘤细胞的增殖活性降低,凋亡明显增加。结论:Notch 3基因沉默可有效抑制白血病细胞的增殖,并促进其凋亡,为急性T淋巴细胞白血病相关基因功能研究及靶向性治疗探索了一种新策略。
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| 关 键 词： | Notch3 survivin启动子 急性T淋巴细胞白血病 |
Effect of Notch 3 silencing on apoptosis and proliferation of acute T-cell lymphoblastic leukemia cells |
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| XIANG Jie,OUYANG Yongri,CUI Ying,LIN Fang,REN Jihong,LONG Min,CHEN Xi,WEI Junxia,ZHANG Huizhong. Effect of Notch 3 silencing on apoptosis and proliferation of acute T-cell lymphoblastic leukemia cells[J]. Journal of Modern Oncology, 2011, 19(8): 1482-1487. DOI: 10.3969/j.issn.1672-4992.2011.08.03 |
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| Authors: | XIANG Jie OUYANG Yongri CUI Ying LIN Fang REN Jihong LONG Min CHEN Xi WEI Junxia ZHANG Huizhong |
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| Affiliation: | XIANG Jie,OUYANG Yongri,CUI Ying,LIN Fang,REN Jihong,LONG Min,CHEN Xi,WEI Junxia,ZHANG Huizhong Department of Clinical Laboratory and Research Center,Tangdu Hospital,Fourth Military Medical University,Xi'an 710038,China |
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| Abstract: | Objective:To explore the effects of silencing of Notch 3 on the development of T-cell acute lymphoblastic leukemia.Methods: We investigated the effect of Notch 3 silencing on SupT1 cells using short hairpin RNA(shRNA) driven by survivin promoter.Results: shRNA targeting Notch 3 could down-regulate the expression of Notch 3 effectively.Down-regulating expression of Notch 3 correlated with a significant apoptosis and a proliferation inhibition.Conclusion: Notch silencing by shRNA driven by surviving promoter ... |
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| Keywords: | Notch 3 |
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