QTY06对LPS引起的大鼠慢性气道炎症及MUC5ac表达的影响

目的：观察合成药QTY06对大鼠气管滴入脂多糖（LPS）诱发的慢性气道炎症及黏蛋白表达的影响。方法：以LPS气管滴注制作慢性支气管炎症模型，观察3周后的支气管肺泡灌洗液中磷脂和白细胞总数的变化、病理学改变、免疫组化特点以及QTY06对其的影响。结果：合成药QTY06给药后能使BALF中的磷脂含量明显增加、中性粒细胞和淋巴细胞百分率及白细胞总数降低。病理切片观察发现QTY06给药组与模型组相比气管炎、支气管炎和肺间质炎严重程度明显降低，上皮损害减轻，炎症细胞浸润减少，阳性杯状细胞数下降。QTY06使气管和细支气管上皮内黏蛋白MUC5ac表达的阳性染色区域和程度明显减轻，通过图像分析系统测得的光密度值和黏蛋白面积％均明显低于模型组。结论：QTY06对LPS诱发的大鼠慢性气道炎症反应有抑制作用，能增加肺表面活性物质磷脂量，并能抑制气道黏蛋白高分泌。

Effect of QTY06 on lipopolysaccharide-induced chronic airway inflammation and MUC5ac secretion in rats

OBJECTIVE: To investigate the effect of synthetic drug QTY06 on chronic airway inflammation and mucoprotein expression induced by intratracheal (i.t) instillation of lipopolysaccharide (LPS). METHODS: Chronic airway inflammation was induced by i.t instillation of LPS in rats. Phospholipids content and the number of leucocytes in bronchoalveolar lavage fluid (BALF), pathological and immunochemical changes were examined 3 weeks after LPS instillation. The effect of QTY06 on chronic airway inflammation was observed. RESULT: After treatment with QTY06, phospholipids in BALF was significantly increased, and the percentages of neutrophils and lymphocytes were decreased as well as the total number of leucocytes. Compared with the model group, pathological examination showed that tracheitis, bronchitis and pulmonary interstitial inflammation in QTY06 groups were significantly attenuated; epithelial damage was alleviated, infiltration of inflammatory cells reduced and the number of goblet cells decreased. QTY06 significantly decreased MUC5ac expression in trachea and bronchiole epithelium, and reduced the optical density and mucins area (%) as detected by image analysis in rats with chronic airway inflammation. Conclusion: QTY06 can reduce and inhibit the chronic airway inflammation induced by LPS in rats, and increase the content of phospholipids in pulmonary surfactant and inhibit the hypersecretion of airway mucins.