螺旋神经节神经元原代培养及其免疫细胞化学鉴定

目的 建立成体哺乳动物耳蜗螺旋神经节神经元（ＳＧＮ）体外培养的细胞模型。方法 对出生后７ｄ的Ｗｉｓｔａｒ大鼠的螺旋神经节细胞进行解离与分散培养。用荧光显微镜与倒置相差显微镜观察原代培养ＳＧＮ细胞的活力以及生长与分化过程。应用链霉卵白素过氧化物酶（ＳＰ）方法和抗神经丝蛋白单克隆抗体（ＮＦＰ－ＭｃＡｂ）对ＳＧＮ进行免疫细胞化学染色鉴定。结果 幼龄Ｗｉｓｔａｒ大鼠的ＳＧＮ在体外条件下，可良好存活并有正常

Primary culture and immunocytochemical identification of spiral ganglion neurons in postnatal Wistar rats]

OBJECTIVE: To create a cytological model of spiral ganglion neurons in postnatal mammals in vitro. METHODS: The primary culture of spiral ganglion neurons (SGN) was carried out with seven-day postnatal Wistar rats. The process of cellular growth and differentiation of SGN were observed by fluorescent microscope and inverted/phase contrast microscopy. Immunocytochemical identification was performed on the cultured SGN of Wistar rats by methods of S-P and the monoclonal antibody of neurofilament protein (NFP-McAb). RESULTS: The present work showed that the dissociated SGN of Wistar rat could survive well and had a normal phenotypic differentiation in vitro. The cell amount and surviving period of SGN were able to meet the requirements of cytological experiments. The stable neuronal plasticity of SGN existed in the postnatal mammal under the present experimental conditions. CONCLUSION: This method extends the material sources of inner ear tissue culture and provides a useful approach to the neurobiologic studies on the peripheral auditory system in vitro.