Characteristics of the microbial community in rhizosphere of <Emphasis Type="Italic">Camptotheca acuminata</Emphasis> cultured with exotic invasive plant <Emphasis Type="Italic">Eupatorium adenophorum</Emphasis> |
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Authors: | Zu YuanGang Gao ChongYang Wang WenJie Yang FengJian Liu Ying Wang Min Zhao YangGuo |
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Affiliation: | 1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China 2. School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China |
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Abstract: | The traditional culture-dependent plate counting and culture-independent small-subunit-ribosomal RNA gene-targeted molecular
techniques, Single-Strand Conformation Polymorphism (SSCP) and terminal Restriction Fragment Length Polymorphism (tRFLP) combined
with 16S rDNA clone library were adopted to investigate the impacts of secretion from Camptotheca acuminata (abbreviated to Ca) roots on the quantities and structure of eukaryotic microbes and bacteria in the rhizosphere, and the
possibility that Ca controls exotic invasive plant Eupatorium adenophorum (Ea). The counting results indicated that the number of bacteria increased in turn in rhizospheres of Ea, Ca-Ea mixed culture
and Ca, while that of eukaryotic microbes decreased. PCR-SSCP profiles showed eukaryotic microbial bands (corresponding to
biodiversity) in rhizosphere of Ea were more complex than those of Ca and CE. Meristolohmannia sp., Termitomyces sp. and Rhodophyllus sp. were the dominant populations in the rhizosphere of Ca. Bacterial terminal restriction fragments (TRFs) profiles showed
no difference among three kinds of rhizospheres, and the sequences of the 16S rDNA clone library from Ca rhizospheres were
distributed in 10 known phyla, in which phylum Proteobacteria were the absolute dominant group and accounted for 24.71% of
the cloned sequences (δ-Proteobacteria accounted for up to 17.65%), and phyla Acidobacteria and Bacteroidetes accounted for
16.47% and 10.59% of the cloned sequences, respectively. In addition, high performance liquid chromatography detected a trace
amount of camptothecin and hydroxycamptothecin in the rhizospheric soil of Ca and CE, but examined neither camptothecin nor
hydroxycamptothecin in rhizospheric soil of Ea. Therefore, invasion and diffusion of Ea evidently depended on distinguishing
the eukaryotic community structure, but not on that of the bacterial pattern. Ca was able to alter the eukaryotic community
structure of invasive Ea by secreting camptothecin and hydroxycamptothecin into rhizospheres, and may benefit the control
of overspread of Ea. This study provided theoretical evidence for rhizospheric microbial aspects on substituting Ca for Ea. |
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Keywords: | Camptotheca acuminata rhizospheric microbe Eupatorium adenophorum Single-Strand Conformation Polymorphism (SSCP) terminal Restriction Fragment Length Polymorphism (tRFLP) 16S rDNA clone library |
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