| 浣熊SRY-HMG box的克隆和序列分析 |
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| 引用本文： | 杨晓娟,杨玉华,张义正,陈红卫,费立松,宋云芳,何光昕.浣熊SRY-HMG box的克隆和序列分析[J].动物学报,2002,48(3):425-428. |
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| 作者姓名： | 杨晓娟 杨玉华 张义正 陈红卫 费立松 宋云芳 何光昕 |
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| 作者单位： | 1. 四川大学生命科学学院分子生物学实验室,成都,610064
2. 成都动物园,成都,610064 |
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| 基金项目： | 四川省成都市大熊猫繁育研究基金，， |
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| 摘 要： | We amplified the 197 bp HMG box sequence by using Polymerase Chain Reaction (PCR) with primers according to the known SRY sequence from male raccoon genomic DNA, and then cloned and sequenced. The nucleic acid and amino acid sequence comparison between raccoon and other mammals revealed high conservation of the SRY HMG box in mammals (about 80%), which implied that the DNA binding activity was crucial to mediate the action of SRY in sex determination. The variation of raccoon HMG box sequence mainly occurred on purine by replacement and missense mutation, which implicated that only the HMG box protein with advanced structure had efficient activity. The homology and difference between mammals HMG box was accorded to the evolution systematic tree.
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| 关 键 词： | 浣熊 克隆 SRY基因 HMG－box PCR 序列分析 性别分化 |
CLONING AND SEQUENCE ANALYSIS OF SRY-HMG BOX FROM RACCOON |
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| Abstract: | |
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| Keywords: | Raccoon ( Procyon lotor ) SRY HMG box PCR sequence analysis |
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