HPLC法同时测定人参皂苷Rb1、Rc、Rd、Rg3、CK和Rh2

目的:建立高效液相色谱法同时测定人参皂苷Rb1、Rc、Rd、Rg3、CK和Rh2的方法.方法:采用ODSC18(4.6 mm×150 mm)色谱柱,流动相乙腈-0.05%磷酸水,梯度洗脱,流速1 Ml/min,检测波长203 nm,柱温35 ℃.结果:人参皂苷Rb1、Rc、Rd、Rg3、CK和Rh2分离效果良好,线性关...

Qualitative and Quantitative Analysis of Ginsenoside Rb1, Rc, Rd, Rg3 ,CK and Rh2 Simultaneously by HPLC

Objective：To determine the content of ginsenoside Rbl, Rc, Rd, Rg3, CK and Rh2 simultaneously by HPLC. Methods：The samples were separated on an ODS C18 （4.6 mm × 150 mm） column using aeetonitrile-0.05 % phosphoric acid solution as the mobile phase, with the gradient elution at a flow of 1.0 mL/min and 35℃ column temperature. The wavelength of the detector was set at 203 nm. Results ： A good separation effect can be reached for ginscnoside Rb1 , Re, Rd, Rg3, CK and Rh2 which showed a good linear relationship between the peak area and the concentration of standard samples. The relative standard deviation （RSD） is good to meet the standard of pharmacopoeia of PRC. Conclusion：The method is simple, accurate, with a good reproducibility. It can be applied to qualitative and quantitative analysis of ginsenoside Rb1, Re, Rd, Rg3, CK and Rh2.