转基因水稻表达的Bt蛋白对拟环纹豹蛛(Pardosa pseudoannulata)生长发育的影响

为明确转基因水稻表达的Bt蛋白沿捕食性食物链的传递及其对生态系统中较高营养级生物的影响,在实验条件下(28±0.5 ℃,R.H=80%,L∶D=12h∶12h)以田间优势自然天敌拟环纹豹蛛为对象,以取食转Cry1Ab/Cry1Ac基因水稻汕优63(简称Bt水稻)叶片24 h的稻纵卷叶螟幼虫为猎物饲养拟环纹豹蛛,并测定了...

Effects of Bt Protein Expressed by Transgenic Rice on the Development of Wolf Spider(Pardosa pseudoannulata)

In order to appraise Bt protein expressed by transgenic rice transfer and affect the third trophie level creature on the food chain, The natural enemy wolf spider （Pardosa pseudoannulata） , which dominates paddy field, was used in this experiment（28±0.5℃ ,R. H = 80 % ,L：D = 12h：12h）. Meanwhile, Bt content of the larvae of Rice case worm （ Cnaphalocrocis medinalis） and wolf spider in different treating time was quantified. The results showed that a certain amount of Bt protein was measured from Rice case worm treated for 12 h,24 h,48 h,60 h and spiders for 2 d,4 d ,6 d ,8 d, 10 d,respeetively,and the Bt content of spider was more than that of Rice ease worm, but control group had no Bt trace; significantly higher development and lower mature spiders weight were observed in the wolf spider fed on Bt rice compared with control treatment（p 〈 0.01 ）; longer spiders life-span than that of management group（p 〈 0.05） though the spiders molting has no significant difference （p 〉 0.05 ）. These results demonstrated the Bt protein expressed by transgenic rice transfer to the third trophlc level on food chain, while these protein affect wolf spiders development in a certain extent.