姜黄素对大鼠胸主动脉瘤Bcl-2和Bax表达的影响

目的 探讨姜黄素对大鼠胸主动脉瘤形成过程中Bcl-2和Bax表达的影响。方法 将30只成年雄性Wistar大鼠随机分为3组，即对照组、动脉瘤组和姜黄素治疗组。采用氯化钙（CaCl2）诱导法制备胸主动脉瘤模型,术后4周取材。HE染色及地衣红染色观察胸主动脉组织学改变；免疫组织化学和Western blotting方法检测动脉瘤壁Bcl-2和Bax的表达。结果 姜黄素可明显抑制胸主动脉的扩张。与对照组比较，动脉瘤组Bax表达水平明显升高，Bcl-2表达水平降低，姜黄素能有效降低动脉瘤中Bax的表达，升高Bcl-2表达水平。 结论 姜黄素可以促进胸主动脉瘤Bc1-2蛋白的表达，抑制Bax蛋白的表达，降低Bax/Bcl-2的比值。

Effects of curcumin on the expression of Bcl-2 and Bax in rat thoracic aortic aneurysm

Objective Curcumin can suppress the development of abdominal aortic aneurysms by inhibiting inammation. The objective of this study is to investigate the effect of curcumin on the expression of Bcl-2 and Bax during rat thoracic aortic aneurysm formation. Methods Thirty adult male Wistar rats were randomly divided into three groups: control group, aneurysmal group and curcumin group. The thoracic aortic aneurysm model induced by CaCl₂ was made, followed by daily oral gavage with 100 mg/kg curcumin or vehicle alone. The thoracic aortic segments were collected at 4 weeks after surgery. HE staining and orcein staining were used to observe the histological changes in the thoracic aorta. Immunohistochemistry and Western blotting were performed to detect the expressions of Bcl-2 and Bax in the wall of aneurysm. Results Curcumin significantly inhibited CaCl₂ -induced expansion of thoracic aorta, along with structural preservation of medial elastin fibers. Compared with the control group, the expression of Bax was higher, and the expression level of Bcl-2 was lower in the aneurysm group. Curcumin effectively reduced the expression of Bax, and increased Bcl-2 expression level in the aneurysm. Conclusion Our study shows that curcumin can promote the expression of Bcl-2 protein, inhibit the expression of Bax protein, and decrease the ratio of Bax/Bcl-2 in thoracic aortic aneurysms.