大鼠腹腔巨噬细胞表达促肾上腺皮质激素释放激素的实验研究

目的 观察促肾上腺皮质激素释放激素(CRH)在大鼠腹腔巨噬细胞的表达变化规律.方法 体外培养大鼠腹腔巨噬细胞,采用RT-PCR及ELISA技术检测正常及脂多糖(LPS)、佛波脂(PMA)刺激后,细胞CRH mRNA转录水平及CRH多肽合成水平.结果 RT-PCR结果显示,正常大鼠腹腔巨噬细胞内有一定水平的CRH mRNA,LPS及PMA刺激后,细胞内CRH mRNA水平均显著增高(P＜0.01).在多肽水平,正常大鼠腹腔巨噬细胞可表达低水平的CRH[约(0.07±0.01)ng/107cells],LPS及PMA促进了CRH多肽的合成(P＜0.01).结论 正常大鼠腹腔巨噬细胞可合成表达CRH,其水平在活化后大鼠腹腔巨噬细胞显著上调.

Expression of corticotrophin-releasing hormone in rat peritoneal macrophages

Objective To investigate the expression of corticotrophin-releasing hormone in rat peritoneal macrophages.Methods Rat peritoneal macrophages were harvested from Wistar rats and cultured in DMEM with 10% fetal bovine serum.RT-PCR was used to observe CRH mRNA in normal cultured cells and cells stimulated by LPS and PMA.ELISA was used to observe CRH peptide in normal cultured cells and cells stimulated by LPS and PMA.Results The RT-PCR data suggests that CRH mRNA existed in normal rat peritoneal macrophages and its level was up-regulated by LPS and PMA(P<0.01).The data of ELISA suggests that CRH peptide also existed in normal rat peritoneal macrophage and was promoted by LPS and PMA(P<0.01).Conclusion CRH existes in normal rat peritoneal macrophage and its expression is up-regulated in activated cells.