| HPLC-DAD结合交替三线性分解二阶校正法测定荔枝果肉中游离植物甾醇 |
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| 引用本文： | 项雷文,陈国泰,翁佳敏,陈文韬,汪少芸.HPLC-DAD结合交替三线性分解二阶校正法测定荔枝果肉中游离植物甾醇[J].食品科学,2015,36(24):172-176. |
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| 作者姓名： | 项雷文 陈国泰 翁佳敏 陈文韬 汪少芸 |
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| 作者单位： | 1.福建师范大学福清分校海洋与生化工程学院，福建 福清 350300；;
2.福州大学生物科学与工程学院，福建 福州 350108 |
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| 基金项目： | 福建省省级重点学科“食品科学与工程”建设项目（闽教高[2012]48号）；福建省区域科技重大项目（2014N3005） |
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| 摘 要： | 为快速测定荔枝果肉中游离植物甾醇含量,建立高效液相色谱-二极管阵列检测器结合基于交替三线性分解算法的二阶校正方法。基本色谱条件:岛津ODS-SP柱(150 mm×4.6 mm,5μm),流动相体积分数95%乙腈溶液,检测波长范围为190~340 nm,柱温30℃,流速1.0 m L/min,进样量20.0μL。结果表明,‘兰竹’和‘乌叶’荔枝样品中菜油甾醇含量分别为24.1μg/g和27.4μg/g,两种荔枝样品豆甾醇含量分别为25.8μg/g和26.7μg/g,菜油甾醇和豆甾醇加标回收率分别为(95.25±0.78)%和(96.83±1.01)%。所建立的方法简单、准确、灵敏度高且可靠,可用于检测荔枝果肉中游离植物甾醇含量。
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| 关 键 词： | 高效液相色谱 二极管阵列检测器 交替三线性分解 二阶校正 植物甾醇 |
Determination of Free Phytosterols in Litchi Pulp by HPLC-DAD Coupled with Second-Order Calibration Based on Alternating Trilinear Decomposition |
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| XIANG Leiwen,CHEN Guotai,WENG Jiamin,CHEN Wentao,WANG Shaoyun.Determination of Free Phytosterols in Litchi Pulp by HPLC-DAD Coupled with Second-Order Calibration Based on Alternating Trilinear Decomposition[J].Food Science,2015,36(24):172-176. |
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| Authors: | XIANG Leiwen CHEN Guotai WENG Jiamin CHEN Wentao WANG Shaoyun |
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| Affiliation: | 1. School of Ocean Science and Biochemistry Engineering, Fuqing Branch of Fujian Normal University, Fuqing 350300, China;
2. College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China |
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| Abstract: | A rapid method to quantify free phytosterols in litchi pulp was established with high performance liquid
chromatography with diode array detection (HPLC-DAD) coupled with second-order calibration method based on
alternating trilinear decomposition (ATLD) algorithm. The chromatographic conditions were simplified by applying secondorder
calibration method. The mobile phase was 95% aqueous acetonitrile (V/V) at a flow rate of 1.0 mL/min. The injection
volume was 20.0 μL. The temperature of inertsil ODS-SP (150 mm × 4.6 mm, 5 μm) column was set at 30 ℃. The detection
wavelengths were 190–340 nm. The average spiked recoveries of campesterol and stigmasterol in litchi pulp were (95.25 ±
0.78)% and (96.83 ± 1.01)%, respectively. The pulp of “Lanzhu” and “Wuye” litchi was detected to contain 24.1 and 27.4 μg/g
campesterol, and 25.8 and 26.7 μg/g stigmasterol, respectively. The newly established method is simple, accurate, highly
sensitive and reliable, and can be used to determine free phytosterols in litchi pulp. |
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| Keywords: | high performance liquid chromatography (HPLC) diode array detector (DAD) alternating trilinear decomposition (ATLD) second-order calibration phytosterols |
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