分离胶采血管对化学发光分析技术测定血清孕酮的影响

目的:探讨分离胶采血管对孕酮检测的影响程度,分析孕酮含量在普通血清采血管和分离胶采血管内随时间变化的趋势。方法:采集患者静脉血标本,分别置于普通血清采血管和分离胶采血管内,离心后采用免疫分析系统检测两种采血管内的血清孕酮含量,并根据孕酮含量的不同将其分为低值组(<10 ng/ml)、中值组(10~20 ng/ml)和高值组(>20 ng/ml)后保存在2~8℃条件下,并在第24 h、48 h和72 h重新进行检测。比较两种采血管对孕酮检测的影响。结果:两种真空采血管内孕酮含量在72 h内随时间变化无明显差异。两种管间孕酮含量在低值组(<10 ng/ml)中无差异,而中值组(10~20 ng/ml)和高值组(>20 ng/ml)分离胶采血管中孕酮检测结果低于普通采血管,其差异有统计学意义(Z=-2.580,Z=-2.667;P<0.05)。结论:孕酮含量在两种真空采血管内2~8℃保存条件下,72 h内可以均保持稳定,但使用分离胶采血管采集血液可使血清孕酮测定值偏低,不含分离胶的普通采血管中血清检测孕酮含量普遍高于分离胶采血管。

The influence of blood collection tube with separation gel on detecting serum progesterone by chemiluminescence analysis technique

Objective: To investigate the effect of blood collection tube with separation gel on progesterone detection, and analyze the trend of progesterone content in two tubes with the change of time. Methods: Venous blood samples of patients were collected and were divided into common serum blood collection tube and blood collection tube with separation gel. After centrifugation, the serum progesterone content was detected by using immunoassay system. All of tubes were divided into 3 groups as progesterone content: low-level group(<10 ng/ml), median group(10~20 ng/ml) and high-level group(>20 ng/ml). And stored temperature of them were 2~8℃, and then they were retested at 24 th h, 48 th h and 72 th h since the first detection. The effects of two blood collection tubes on progesterone detection were compared. Results: There was no significant difference in progesterone content with the change of time between three time point in each kind of blood tube. There was no significant difference in the progesterone content of lowlevel group(<10 ng/ml) between the two kinds of tubes, while the progesterone content of median group(10~20 ng/ml) and high-level group(>20 ng/ml) of blood collection tube with separation gel were significantly lower than these of common blood collection tube(Z=-2.580, Z=-2.667, P<0.05). Conclusion: Progesterone content is kept in stability in both tubes within 72 h at 2~8℃, but the blood collection tube with separation gel can reduce the measured value of progesterone. And the serum progesterone content of common blood collection tube without separation gel is generally higher than that of blood collection tube with separation gel.