幽门螺杆菌临床常用检测方法的比较

目的通过对几种幽门螺杆菌检测方法的灵敏性和特异性进行比较,为临床提供一种检测幽门螺杆菌的最佳方案。方法在胃窦大弯侧近幽门2cm处取活组织并抽血2mL,采用快速尿素酶试验法、组织染色法、13C-尿素呼气试验、ELISA法进行幽门螺杆菌测定。结果快速尿素酶试验法、组织银染法、13C-尿素呼气试验、ELISA法的灵敏度分别为84·6%、95·6%、97·8%、89·0%,特异性分别为77·2%、78·9%、88·2%、75·6%。结论4种方法中以13C-尿素呼气试验灵敏性和特异性最高。快速尿素酶法简单、易行、价廉;组织学染色准确率较高;血清HP抗体测定和13C-尿素呼气试验为非侵入性,准确率也较高,患者依从性好。

Comparison of Commonly Used Clinical Methods for the Detection of H. pylori

Objective It was reported that H.pylori is one of the high risk factors in chronic gastritis, ulcer, and gastric cancer, The aim of this study is to provide a useful method for detecting H. pylori. Methods In 150 patients H.pylori was detected with ureasetesting, warthin-starry histology, ELISA technique, and ~ 13 C-breathtest-instructions. Results The sensitivities of the four methods are: ureasetesting (84.6%), histology (95.6%), ~ 13 C-breathtest-instructions (97.8%), ELISA technique(89.0%). The speciticities of the four methods are urease testing (77.2%), histology (78.9%), ~ 13 C-breathtest-instructions (88.2%), ELISA technique(75.6%) respectively. Conclusion Histology are the method with best accuracy for the detection of H.pylori.