癌痛消诱导大鼠移植性肝癌细胞凋亡的实验研究

目的:研究癌痛消颗粒对大鼠移植性肝癌的抑瘤作用以及对肿瘤细胞凋亡的影响,探讨该药抗肝癌作用的机理。方法:采用walker-256瘤株建立SD大鼠的移植性肝癌的模型,分为癌痛消高、中、低剂量组,5-Fu组,蒸馏水组,连续用药14天。停药24h,处死大鼠,取出瘤块,称重,计算抑瘤率,应用流式细胞仪检测细胞凋亡率。结果:癌痛消低、中、高治疗组均有抑瘤作用,抑瘤率分别为12.4%、32.4%、45.7%;癌痛消低、中、高剂量组均可诱导肝癌细胞凋亡,与蒸馏水组比较,癌痛消高、中剂量组差异均具有显著性意义;癌痛消高、中剂量组能改善模型大鼠体重变化。癌痛消各剂量组较蒸馏水组和5-FU组,一般情况较好,症状较轻。结论:癌痛消能改善移植性肝癌大鼠的生存质量,抑制大鼠移植性肝癌细胞生长,其作用机制可能与诱导肝癌细胞凋亡有关,其作用强度可能与剂量呈正相关。

Experimental Research on Apoptosis of Rat Transplantated Hepatocellular Carcinoma Cell Induced by AiTongXiao Granule

Objective:To observe the effect of AiTongXiao Granule(ATX)on inhibiting tumorous action as well as cellular apoptosis in the rats with transplanted liver cancer,and then to explore its corresponding mechanism.Methods:Male spradue-Dawley rats healthy rats,which were implanted liver tumors(Walker-256 carcinosarcoma),were divided into distilled water group,5-Fu group,mintute dosage group of ATX,medium dosage group of ATX,large dosage group of ATX,and then were treated 14days,to observe every group rat weight change,ordinary circumstances as well as the symptom during before and after using the medicine,and compared with the normal group.Then 24hours afterwards,the rats were killed to take out the tumor so as to detemine the cellular apoptosis by FCM method.Meanwhile,the tumor was surveyed weight and then calculated inhibiting tumorous ratio.Results:Three group of ATX had effect on inhibiting tumor and the tumor inhibiting rate was 12.4%,32.4%and 45.7% respectively;The cellular appoptosis was deduced in the three group of ATX,the difference between the distilled water group,medium and large dosage group of ATX has significance meaning;The medium and large dosag group of ATX can improve modal rates' weight change.Compared to the distilled water group and the mormal group,every group of ATX ordinary circumstances is better,the symptom is lighter.Conclusion:ATX can improve the rat with transplantated hepatocellular carcinoma survival mass,and it has the effect on inhibiting tumorous in the rats with transplanted cancer.Its mechanism is probably related to deducing the cellular apoptosis in the rats with transplanted liver cancer.The intensity of effect is Possibly in direct ratio to the dosage of ATX.