消瘀通络散对乳腺增生大鼠乳腺组织形态与内分泌的影响

目的:观察自拟消瘀通络散对乳腺增生大鼠乳头直径、高度,血清E2/P、PRL、T水平以及乳腺组织形态的影响,探讨其治疗乳腺增生的作用机理。方法:将40只Wistar大鼠随机分为正常组、模型组、中药组和西药组4组。采用己烯雌酚联合黄体酮肌注的方法进行造模。中药组给予自拟消瘀通络散50mg/d,西药组予三苯氧胺1.8mg/kg,均灌胃治疗,1次/d,共30d。于治疗前后测量各组大鼠第2对乳头直径、高度,测定血清E2、P、PRL、T水平,计算E2/P数值,取大鼠第2对乳房常规HE染色,光镜下观察组织形态表现。结果:治疗后乳头直径、高度比较,中药组和西药组均低于模型组(P0.05);2组之间比较,中药组低于西药组(P0.05)。血清各激素水平比较,中药组和西药组血清E2/P、PRL、T含量均低于模型组(P0.05);中药组与西药组相比无显著性差异(P0.05)。病理学比较,中药组和西药组大鼠乳腺组织病理表现相似,与模型组比较改善明显,与正常组相近。结论:自拟消瘀通络散具有缩小模型大鼠乳头直径、高度,改善内分泌紊乱状态和乳腺组织病理形态的作用。

Effects of Xiaoyu Tongluo San on breast morphology and endocrine in rats with hyperplasia of mammary glands

Objective: To observe the effect of Xiaoyu tongluo san on the second pair of breast nipple diameter,height and serum E2/P ratio,PRL,T levels、breast tissue of the Mammary Gland Hyperplasia rats model,discuss the therapeutic mechanism of HMG.Methods: 40 Wistar rats were divided into 4 groups randomly:normal group,model group,TCM group and western medicine group.Combined diethylstilbestrol with progesterone by intramuscular injection to copy breast Hyperplasia rats model.TCM group were treated with self-Xiaoyu tongluo san 50mg/d,while western medicine group were treated with TAM 1.8mg/kg,gavage was both applied,1 time/d,30 days totally.Detected the second pair of breast nipple diameter,height and serum E2/P ratio,PRL,T levels before and after treatment,calculate E2/P ratio,The second pair of nipple were removed aseptically,stained HE,breast forms was observed under microscope.Results: After treatment,comparision of the second breast nipple diameter and height: TCM group and western medicine group were lower than model group(P<0.05);TCM group were lower than western medicine group(P<0.05).Comparision of serum levels of the hormone: TCM group and western medicine group were lower than model group(P<0.05),compared TCM group with western medicine group,the difference was not statistically significant(P>0.05).Comparision of breast tissue pathological forms: The pathological forms in TCM group were similar to those in TAM group,but they were both better than that in model group,and similar to the normal group.Conclusion: The self-Xiaoyu tongluo san has effects on reducing nipple diameter,height and regulating the dysfuction of serum E2/P ratio,PRL,T and improve the breast tissue pathological forms of Mammary Gland Hyperplasia rats.