葛根素对胃癌多药耐药的逆转实验研究

目的观察葛根素注射液对人胃癌耐药细胞系SGC7901/VCR的影响。方法采用MTT法进行药敏实验,并通过流式细胞仪检测细胞周期变化、胞浆内药物浓度,荧光显微镜显示荧光强度,免疫细胞化学染色法显示耐药细胞中多药耐药相关蛋白(MRP)和P-糖蛋白(P-gp)的分布,观察化疗药(包括5-FU、VCR、DDP、ADM)和/或葛根素对胃癌裸鼠原位移植瘤的抑瘤率。结果葛根素注射液能够逆转SGC7901/ VCR对5-FU的耐药性,与SGC7901/VCR比较S期和G_2+M期增加,G_1期减少,细胞内的阿霉素荧光强度明显增强,MRP和P-gp的表达明显减弱,葛根素和5-FU联合应用对裸鼠胃癌的抑瘤率明显高于单纯应用5-FU组。结论葛根素注射液能够逆转SGC7901/VCR的多药耐药性,其机理可能是促使MRP和P-gp蛋白表达减少。

Experiment on Multidrug-Resistance Reversing Effect of Puerarin Injection in Patients with Gastric Carcinoma

Objective To investigate the effect of puerarin injection on human drug-resistant cell line SGC7901/VCR.Methods The drug sensitivity test was carried out with WTT assay,the change of cell cycle and concentration of drug in cytoplasma was measured by flow cytometric method,the distribution of muhidrug-resistance relevant protein(MRP)and P-glucoprotein(PGP)in drug-resisent cells were determined by immunohistochemical stainning,and the tumor inhibitory rate of puerarin and chemotherapeutic agents,including 5-FU,VCR,DDP and ADM,in nude rats with transplanted gastric carcinoma in situ was observed.Results Puerarin injection could re- verse the drug resistance of SGC7901/VCR to 5-FU,the proportion of cells in S phase and G_2 plus M phase in- creased,and that in G_1 phase decreased as compared to the untreated control.In the same time,the fluorescent in- tensity of adriamycin in cells was significantly increased,the expression of MRP and P-GP were significantly de- creased.The tumor inhibitory rate in nude rats with gastric carcinoma treated by combined puerarin and 5-FU was higher than that only treated with 5-FU alone.Conclusion Puerarin injection could reverse the multidrug resistance of SGC7901/VCR,its mechanism may relate with its action in decreasing protein expression of MRP and P-GP.