应用大孔吸附树脂分离纯化香椿叶总黄酮

 测定了6种大孔吸附树脂对香椿叶中总黄酮的吸附解吸性能，确定了大孔吸附树脂吸附分离香椿叶总黄酮的工艺条件。结果表明LSA-10树脂对香椿叶总黄酮有良好的吸附解吸性能，其最佳吸附分离的工艺条件为：上柱液总黄酮质量浓度0.653 mg/mL，pH 4.0~5.5，流速240 mL /h，饱和吸附量440~480 mL，用400 mL 50%乙醇以流速80 mL /h解吸。经4次上柱吸附解吸，香椿叶总黄酮纯度可达75.2%。

Separation and Purification of Total Flavonoids from Toona sinensis Leaves by Macroporous Resins

In order to establish the technology for separating and purifying total flavonoids from Toona sinensis leaves with macroporous resin, 6 types of macroporous resins were selected to be investigated. The results showed that LSA-10 was the best for separating and purifying total flavonoids in the following conditions: the loaded concentration of total flavones 0.653 mg/mL, pH 4.0-5.5, the flow rate 240 mL/h; amount of saturated adsorption for total flavones 440-480 mL; 400 mL 50% ethanol as appropriate the eluting reagent, eluting rate 80 mL/h. The purity of total flavonoids purified by LSA-10 was 75.2% when purified four times under the optimal conditions.