| 散发性结直肠癌FHIT基因杂合性缺失与临床病理特征的关系 |
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| 引用本文： | 雷大钊,陈新岐,白剑. 散发性结直肠癌FHIT基因杂合性缺失与临床病理特征的关系[J]. 临床医学工程, 2010, 17(5): 33-35 |
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| 作者姓名： | 雷大钊 陈新岐 白剑 |
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| 作者单位： | [1]河南省邓州市第一人民医院普通外科,河南邓州474150 [2]广州医学院港湾医院,广东广州510700 |
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| 摘 要： | 目的探讨散发性结直肠癌FHIT基因杂合性缺失与临床病理特征的关系。方法收集散发性结直肠癌新鲜标本及其配对的癌旁正常组织标本63例,选取微卫星位点D3S1300和D3S1234标记FHIT基因的杂合性缺失,采用PCR-单链构象多态性(SSCP)-银染技术检测以上微卫星位点,并结合患者的临床资料(年龄、性别、肿瘤位置、肿瘤分化程度、Dukes分期及有无淋巴结转移)分析二者之间的关系。结果 FHIT基因杂合性缺失的检出率为22.0%,其与肿瘤的分化程度、有无淋巴结转移显著相关(p<0.05),与其他临床病理参数无明显关系(p>0.05)。结论 FHIT基因的杂合性缺失可能在结直肠癌的发生发展中起着重要作用。
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| 关 键 词： | 结直肠肿瘤 FHIT基因 杂合性缺失 PCR-SSCP |
The Relationship between Loss of Heterozygosity of FHIT Gene and Clinical Pathological Characteristics in Sporadic Colorectal Cancer |
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| Abstract: | Objective To study the relationship between loss of heterozygosity(LOH)of FHIT gene and clinical pathological characteristics in sporadic colorectal cancer.Methods To collect 63 cases of fresh sporadic colorectal cancer tissues and matched normal tissues.LOH of FHIT gene were detected at two microsaterllite locis D3Sl300 and D3S1234 by the method of polymerase chain reaction single strand conformation polymorphism(PCR-SSCP)and silver staining.LOH of FHIT gene were analyzed in combination with the clinical data(age,sex,etc).Results The rate of LOH of FHIT gene was 22.0%.LOH of FHIT gene had a significant correlation with tumor differentiation and lymph node metastasis(p<0.05),while no significant correlation with other clinical pathological characteristics(p>0.05). Conclusion LOH of FHIT gene maybe playe an important role in the occurrence and development of sporadic colorectal cancer. |
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| Keywords: | Colorectal neoplasms FHIT gene Loss of heterozygosity PCR-SSCP |
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