解脲支原体检测试剂盒的研制

研制出一种快速、敏感、准确检测解脲支原体试剂盒。方法：筛选出促进解脲支原体生长的促 生长因子，替代新生牛血清。选用多种有效抑菌剂以省去固体培养基鉴定的步骤，并与Ｈａｙｆｌｉｃｋ培养基及无血培 养基进行对比研究。结果：７２小时自制试剂盒与Ｈａｙｆｌｉｃｋ培养基的检出符合率达９８．１％， １６小时内检出率自制 试剂盒、Ｈａｙｆｌｉｃｋ培养基、无血解脲支原体培养基分别为５２．６％、２７．６％、１６．３％，前者明显优于后两种培养基。结 论：自制解脲支原体试剂盒简便、快速、准确，是一种实用的检测试剂盒。

The kit of detection ureaplasma urelyticum

To produce a kit for the rapid sensitive correct detection of ureaplasma urelyticum. Methods: We select a growing factor to take the place of calf serum and effectiveness antibiotics to abridge further detection in solid culturemadium. We compared the kit with Hayflick and no serum culture-medium. Results: The rat of detection accord the kit with Hayflick culture-medium was 98.1% in 72 hour. The rat of detection of the kit Hayflick and no serum culture-medium respectively was 52.6%, 27.6%, 16.3% for ureaplasma urelyticum in 16 hour. Conclusion:The kit of self produce is convenient rapid correct kit for detection of ureaplasma urelyticum.