| 共刺激分子B7h在SY5Y中的表达 |
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| 引用本文： | 刘淑娟,王金龙.共刺激分子B7h在SY5Y中的表达[J].宁波大学学报(理工版),2007,20(1):138-140. |
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| 作者姓名： | 刘淑娟 王金龙 |
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| 作者单位： | 1. 天津医科大学,生物学教研室,天津,300070
2. 天津医科大学,免疫学教研室,天津,300070 |
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| 摘 要： | 探讨B7h在SY5Y中的表达情况,运用逆转录聚合酶链反应(RT-PCT)技术从SY5Y获得B7h基因片段,并采用间接免疫荧光标记方法确证靶分子的表达.经琼脂糖凝胶电泳检测证明,成功获得B7h基因片段;经间接免疫荧光标记方法证明,SY5Y中可表达B7h分子.
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| 关 键 词： | B7h 共刺激分子 SY5Y 共刺激分子 表达 法证 间接免疫荧光标记 电泳检测 琼脂糖凝胶 靶分子 标记方法 基因片段 转录聚合酶链反应 运用 情况 |
| 文章编号： | 1001-5132(2007)01-0138-03 |
| 修稿时间： | 2007-01-15 |
The Expression of Co-stimulatory Molecules B7h in SY5Y |
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| LIU Shu-juan,WANG Jin-long.The Expression of Co-stimulatory Molecules B7h in SY5Y[J].Journal of Ningbo University(Natural Science and Engineering Edition),2007,20(1):138-140. |
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| Authors: | LIU Shu-juan WANG Jin-long |
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| Affiliation: | 1 .Department of Biology, Tianjin Medical University, Tianjin 300070, China; 2.Department of Immunology, Tianjin Medical University, Tianjin 300070, China |
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| Abstract: | The expression of B7h in SY5Y cells is studied. The technology of RT-PCR is used to acquire B7h genetic fragment from SY5Ycells and the expression of target molecule is further proved through marking method of indirect immuno fluorensese. Through agarose gel electrophoresis, it is found that there is a bright band at ! 052 bp, indicating the existence of B7h genetic fragment. The marking method of indirect immuno fluorensese proves that SY5Y cells can be expressed by B7h. |
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| Keywords: | B7h co-stimulatory molecules SY5Y |
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