Oil and fat hydrolysis with lipase fromAspergillus sp. |
| |
Authors: | X Fu X Zhu K Gao J Duan |
| |
Affiliation: | (1) Department of Fine Chemicals Technology, Dalian University of Technology, 116012 Dalian, People’s Republic of China;(2) Microbe Chamber, Shenyang Institute of Applied Ecology, China Academy of Science, 110015 Shenyang, People’s Republic of China |
| |
Abstract: | Hydrolysis of olive oil, soybean oil, mink fat, lard, palm oil, coconut oil, and a hydrogenated, hardened oil with lipase
from anAspergillus sp. has been studied. The lipase had high specific activity (60,000 U/g) and did not show any positional specificity. The
lipase proved to be a more effective catalyst than Lipolase fromA. oryzae, with an optimal activity at 37°C and pH 6.5–7.0. It was activated by Ca2+ but inactivated by organic solvents such as isopropanol and propanone. All substrates examined could be hydrolyzed to corresponding
fatty acids with this enzyme at concentrations of 5–30 U/meq with yields of 90–99% in 2–24 h. The degree of hydrolysis was
almost logarithmically linear with reaction time and occurred in two stages. The lipase was stable and could be repeatedly
recycled for hydrolysis. |
| |
Keywords: | Enzyme concentration enzyme recovery fatty acids lipase lipase activity lipolysis oil and fat percent hydrolysis reaction time specificity |
本文献已被 SpringerLink 等数据库收录! |
|