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中性蛋白酶水解玉米蛋白粉制备可溶性肽最佳条件的研究
引用本文:刘新华,赵晨霞,李治龙,王鹏飞,王晓娟,胡彩香. 中性蛋白酶水解玉米蛋白粉制备可溶性肽最佳条件的研究[J]. 食品科学, 2009, 30(7): 194-196. DOI: 10.7506/spkx1002-6630-200907044
作者姓名:刘新华  赵晨霞  李治龙  王鹏飞  王晓娟  胡彩香
作者单位:1.新疆塔里木大学生命科学学院 2.北京农业职业学院园艺系 3.新疆农业大学食品科学学院
摘    要:通过对酶法水解玉米粉蛋白制备多肽的工艺进行了研究,实验以水解度(DH)和氮溶解指数(NSI)为指标确定了最佳酶解工艺条件。结果表明,在pH7.5、酶解温度50℃、酶与底物比2%、底物浓度5%的条件下酶解4h,可使水解度和氮溶解指数分别达到22.74%和23.86%。

关 键 词:玉米蛋白粉  酶解  优化  
收稿时间:2008-06-26

Optimization of Production Conditions of Soluble Peptides from Corn Gluten Meal Hydrolyzed with Neutral Protease
LIU Xin-hua,ZHAO Chen-xia LI Zhi-long,WANG Peng-fei WANG Xiao-juan HU Cai-xiang. Optimization of Production Conditions of Soluble Peptides from Corn Gluten Meal Hydrolyzed with Neutral Protease[J]. Food Science, 2009, 30(7): 194-196. DOI: 10.7506/spkx1002-6630-200907044
Authors:LIU Xin-hua  ZHAO Chen-xia LI Zhi-long  WANG Peng-fei WANG Xiao-juan HU Cai-xiang
Affiliation:(1.College of Life Science, Tarim University, Alaer 843300, China 2.College of Horticulture, Beijing Vocational College ofAgriculture, Beijing 102442, China 3. College of Food Science, Xinjiang Agricultural University, Urumqi 843001, China)
Abstract:The technology of producing polypeptide from corn gluten meal (CGM) via enzymolysis was studied. With degree of hydrolysis (DH) and nitrogen solubility index (NSI) as evaluation indexes, the optimum production conditions were determined as follows: substrate concentration 5%, concentration ratio of neutral protease to substrate 2%, hydrolysis temperature 50 ℃, pH 7.5, and hydrolysis time 4 h. Under these conditions, DH and NSI are 22.74% and 23.86%, respectively.
Keywords:corn gluten meal  enzymolysis  optimization  
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