高效液相色谱法测定蓝莓果浆中糖的组成和含量

目的：建立高效液相色谱法测定蓝莓果浆中糖的组成和含量的检测方法。方法：超声波水提样品中的糖类，色谱柱为Agilent Zorbax carbohydrate 柱(4.6 × 250mm，5μm)，流动相为乙腈- 水(75:25，Ｖ:Ｖ)，流速为1ml/min。结果：糖类浓度在0.5～10mg/ml 内呈现良好的线性关系，相关系数在0.9989(n=5)以上；精密度实验RSD为0.36%～2.18%(n=5)；平均回收率为91.36%～101.34%。结论：该方法简便快速，结果准确可靠，为测定野生蓝莓果浆中糖组分提供了一种高效快速的测定方法。

Determination of Saccharide Components and Contents in Blueberry Juice by HPLC

Objective: To set up an examination method for determining saccharides in blueberry juice by HPLC. Method: Saccharides were extracted from samples with water using ultrasonic, and then their components and contents were determined by HPLC. Agilent Zorbax carbohydrate (4.6 × 250 mm, 5 μm) column was adopted, mobile phase was acetonitrile-water (75:25, V:V), and the flow rate was 1 ml/min. Results: The calibration curve of saccharide concentrations is well linear in the range of 0.5 to 10 mg/ml, the correlation coefficient is over 0.9989 (n=5), the RSD is 0.36% to 2.18% (n=5) in precision experiment, and the average recovery rate is 91.36% to 101.34%. Conclusion: This method is convenient, rapid and effective for separation and quantitative analysis of saccharides in blueberry juice.