鱼腥草注射液抗内毒素性心肌损伤作用的实验研究

目的:揭示鱼腥草注射液抗内毒素诱导的心肌损伤作用的机制。方法:取SD雄性大鼠24只随机分成正常对照组(NC组,n=6),内毒素组(ET组,n=6),鱼腥草注射液+内毒素组(HHI+ET组,n=6)和单纯鱼腥草注射液组(HHI组,n=6),在Langendorff装置上用Krebs-Henseleit(KH)液对大鼠离体心脏行主动脉逆灌。在相应时点以HR、LVSP、LVEDP、LVDP、+dp/dt_max、-dp/dt_max等6项参数为指标,测定冠脉流出液中的超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及心肌组织一氧化氮(NO)含量、琥珀酸脱氢酶(SDH)活性。结果:与ET组相比,HHI+ET组在相应时点,左心室压差与心率乘积(LVSP-LVEDP)×HR、LVDP、+dp/dt_max恢复百分率明显改善,对内毒素诱导的心肌SOD、SDH活性的降低和MDA、NO含量的升高有明显抑制作用。结论:鱼腥草注射液具有明显的抗内毒素心肌损伤作用,通过降低心肌中NO的量,增强心肌的抗氧化能力,稳定心肌酶活性和膜相结构等途径,提高心肌对内毒素性损伤的抵抗力。

Experimental study on herba houttuyniae injection against myocardial injury induced by endotoxin

AIM: To observe the effects of herba houttuyniae injection on myocardial injury induced by endotoxin and to explore the possible mechanism. METHODS: 24 male SD rats,weghting (250±30) g, were randomly divided into three groups: normal control group(NC, n=6), endotoxin group (ET, n=6), herba houttuyniae injection puls endotoxin group (HHI+ET, n=6) and herba houttuyniae injection group (HHI, n=6). Heart was isolated from rats and perfused on Langendorff apparatus with Krebs-Henseleit (KH) buffer (Saturation 95%+5%CO₂) at a constant pressure (8.33 kPa) and temperture(37 ℃). The activities of superoxide dismutase (SOD) and malondialdehyde (MDA) content of efflux from coronary artery were measured at certain time points (0 min,20 min,50 min,80 min). The ventricular function, the concentration of nitric oxide (NO) and the activity of succinate dehydrogenase (SDH) in myocardial tissue were also observed. RESULTS: Compared with ET group, the percentage recovery of the product of left ventricular pressure difference and heart rate , LVDP, +dp/dt_max were all increased apparently in HHI group. The activity of SOD, SDH and the concentration of MDA, NO were close to normal control group. CONCLUSION: Herba houttuyniae injection protects myocardium against injury induced by endotoxin. It may be related to decrease in the level of NO, the strength of antioxidation, the stability of enzyme activities and the improvement of energy metabolism in myocardium.