神经生长因子低亲合力受体在肝纤维化患者和大鼠肝星状细胞的表达

目的探讨神经生长因子低亲合力受体（P75）在肝纤维化患者和大鼠肝星状细胞（HSCs）的分布及作用机制。方法对四氯化碳（CCL）法制备的肝纤维化大鼠肝组织及肝穿刺获取的肝纤维化患者肝组织，常规石蜡包埋；大鼠离体培养的活化HSCs离心涂片，行免疫组织化学染色，确定P75的表达分布。结果P75在大鼠离体培养的活化HSCs膜，免疫组织化学染色呈阳性，在肝纤维化患者HSCs膜和肝细胞膜，免疫组织化学染色呈阳性，在肝纤维化大鼠HSCs膜和肝细胞膜，免疫组织化学染色呈阳性，在健康人和正常对照大鼠HSCs膜，免疫组织化学染色呈阴性，表明人和大鼠活化的HSCs膜表达P75。结论P75为肝纤维化的治疗提供了新的靶点。

THE EXPRESSION OF P75 IN HEPATIC STELLATE CELLS OF FIBROTIC HUMAN AND RAT LIVER

Objective To study the expression site and the role of low-affinity nerve growth factor receptor(P75) in hepatic stellate cells(HSCs) of fibrotic human and rat liver.Methods Left lobe of liver was removed from CCl-4-induced rats fibrosis model and human fibrotic tissue sample was taken from liver puncture.The tissue specimen was routinely paraffin embedded and expression of P75 was detected by histochemical staining using polyclonal antibody against P75.Additionally,HSCs was cultivated and P75 was immunochemically assayed for P75 binding activity. Results P75 was seen abundantly on cell membrane of the cultured HSCs.In the fibrotic tissue of rat liver as well as human fibrotic liver P75 was also positively expressed.Similarly,P75 was found mainly a hepatocye surface in histochemical staining.P75 was not seen by histochemical staining in the normal tissue of human and rat liver.Conclusion P75 used as a new target for the treatment of hepatic fibrosis.