盐酸吡哆辛在聚烟酸修饰电极上的电化学行为及测定

采用电聚合方法制备了聚烟酸修饰玻碳电极(PNA/GCE),并利用循环伏安法(CV)和差分脉冲伏安法(DPV)考察了盐酸吡哆辛(VB6)在该修饰电极上的电化学行为。结果表明,VB6在PNA/GCE上的氧化峰电流显著提高,电极反应为扩散控制的一电子两质子反应。利用差分脉冲伏安法对VB6进行测定,线性范围为0.08~400μmol/L,VB6的检出限为0.02μmol/L,测定结果的相对标准偏差为3.1%(n=8),加标回收率为95.8%~103.7%。该方法可用于VB6片中VB6含量的测定。

Electrochemical Behavior and Determination of Pyridoxine Hydrochloride at the Poly Nicotinic Acid Modified Electrode

Poly（nicotinic acid） modified electrode （PNA / GCE） was prepared and the electrochemical behavior of pyridoxine hydrochloride（VB6） was investigated at the modified electrode by cyclic voltammetry（CV） and differential pulse voltammetry（DPV）. Results showed that the oxidation cux-rent of VB6 at the PNA / GCE was obviously larger than that at GCE. The oxidation process of VB6 was an irreversible diffusion-controlled process involving one electron and two protons. The liner range between the peak current of DPV and the concentration of VB6 was 0.08-400 μmol/L, and the detection limit was 0.02 μmol/L. The modified electrode showed a good reproducibility with a relative standard deviation of 3.1%（n=8）. The recoveries were between 95.8% and 103.7%. The method can be applied to the analysis of vitamin B6 content in vitamin B6 tablets.