灵芪胶囊对S180荷瘤小鼠p53和bcl-2蛋白表达影响的实验研究

[目的]探讨灵芪胶囊对S180荷瘤小鼠的抑瘤作用及其抑瘤作用机制。[方法]采用体内实验法，于无菌条件下抽取传代7d、生长良好的S180荷瘤小鼠腹水，无菌生理盐水1：3稀释（约含瘤细胞2×10g／L），按0．2mL／只接种于消毒后的小鼠右前肢腋部皮下，制成肿瘤模型，经口灌胃灵芪胶囊11d后，称取小鼠体质量，脱椎处死小鼠，取脾脏及胸腺称质量并计算胸腺指数和脾脏指数。用免疫组化法检测各组凋亡相关基因突变型p53和bcl-2基因的表达。[结果]灵芪胶囊在明显抑制肿瘤生长的同时不降低体质量，在一定剂量对荷瘤动物的免疫器官指数无明显影响，明显优于化疗药物；灵芪胶囊3个剂量组突变型p53基因和bcl-2基因的表达均小于模型组。[结论]灵芪胶囊对S180荷瘤小鼠有明显抑瘤作用，其机制可能与抑制突变型p53基因和bcl-2基因表达有关。实验证明灵芪胶囊不失为一种很有前途的抗癌中药。

Effect of Lingqi-capsule on gene expression of p53 and bci-2 on sarcoma Sin-bearing mice

[Objective] To discuss the antitumor effect of Lingqi-capsule on sarcoma S180-bearing mice. [Methods] In vivo experiment was Adopted and ascites sample from sarcoma S180-bearing mice grew for 7 days in asepsis condition. The sample was diluted for 1：3 with asepsis brine （about included sarcoma cell 2×10^9/mL）, inoculated into skin of right forelegs with 0.2ml per mice for establishing the model of sarcoma S180-bearing mice. After giving Lingqi-capsule for 11 days the immunity organs index and the gene expression of p53 and bcl-2 in each group were compared with the model group. [Results] Lingqi-capsule could obviously inhibit tumor growing without losing of body weight and influencing immune organs. The gene expression of p53 and bcl-2 in each group was less than that of the model group. [Conclusion] Lingqi-capsule can obviously inhibit sarcoma S180 mice growing. The mechanism of action may be correlated with decreasing of p53 and bcl-2 gene expression. The aim of this thesis was looking for a new medicine having effect both raising immunity and antitumor from Chinese herbal medicine. Lingqi-capsule was proved to be a promising medicine by this experiment.