渐危药用植物珊瑚菜胚状体途径再生植株的研究

目的:研究离体条件下珊瑚菜种子休眠的原因,建立胚状体途径再生植株的方法。方法:研究离体条件下胚乳因素和外源激素在解除珊瑚菜种子休眠过程中的作用,以及激素浓度对胚性愈伤组织诱导及胚状体再生植株的影响。结果:保留1/3胚乳的珊瑚菜种子萌发率最高,可以达到31%。而TDZ,6-BA,GA3处理不仅对解除珊瑚菜种子休眠的作用不大,同时容易导致出现畸形苗。在附加1.0 mg.L-1 2,4-D的MS培养基上,胚性愈伤的诱导率可以达到57%。将培养20 d左右胚性愈伤组织转入MS培养基上40 d左右就可分化形成子叶期胚状体,然后再继代培养20 d即可得到再生植株。结论:建立一套有效的珊瑚菜再生植株体系。

Study on plant regeneration from somatic embryos of vulnerable medicinal plant Glehnia littoralis

Objective: To study the cause of the seeds dormancy of Glehnia littoralis in vitro and to establish plant regeneration methods via somatic embryos. Method: The effects of endosperm and exogenous hormone on the seed dormancy breaking of G. littoralis and the effect of hormone concentration on embryonic callus induction and plant regeneration via somatic embryos were observed. Results: The germination rate of the seeds with 1/3 endosperm was the highest which achieved 31%. TDZ, 6-BA and GA3 treatment could not break seed dormancy but easily lead to abnormal seedlings. Embryogenic callus induction rates was up to 57% on MS supplemented with 1.0 mg·L^-1 2,4-D. After 20 days culture, embryogenic calli were transferred to MS medium and cotyledonary embryos were formed in 40 days. The regenerated plants were obtained in 20 days. Conclusion: An effective system of plant regeneration of G. littoralis was established in this study.