| 十六份姜花属花卉种质的SRAP分子标记指纹图谱构建 |
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| 引用本文： | 胡秀,高丽霞,刘念,寇亚平,熊友华,黄邦海.十六份姜花属花卉种质的SRAP分子标记指纹图谱构建[J].北方园艺,2011(10):114-117. |
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| 作者姓名： | 胡秀 高丽霞 刘念 寇亚平 熊友华 黄邦海 |
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| 作者单位： | 仲恺农业工程学院,广东,广州,510225;河池学院,广西,河池,546300;广州市农业技术推广中心,广东,广州,510520 |
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| 摘 要： | 姜花属花卉种质来源广泛,包括栽培种、栽培品种及新育成品系等,从形态上常难以鉴定。该研究在全面收集姜花属主要花卉种质的基础上,采用SRAP分子标记方法,从多态性较好的12对引物中筛选出2对引物,构建了16份姜花属常见花卉种质的分子标记指纹图谱。结果表明:采用SRAP分子标记方法,条带清晰、结果稳定、重复性高,可用于姜花属花卉种质的快速、准确的鉴定。
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| 关 键 词： | 姜花属 花卉种质 SRAP分子标记 指纹图谱构建 |
SRAP Fingerprint Construction of Sixteen Hedychium Ornamental Germplasms |
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| HU Xiu,GAO Li-xia,LIU Nian,KOU Ya-ping,XIONG You-hua,HUANG Bang-hai.SRAP Fingerprint Construction of Sixteen Hedychium Ornamental Germplasms[J].Northern Horticulture,2011(10):114-117. |
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| Authors: | HU Xiu GAO Li-xia LIU Nian KOU Ya-ping XIONG You-hua HUANG Bang-hai |
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| Affiliation: | HU Xiu~1,GAO Li-xia~2,LIU Nian~1,KOU Ya-ping~1,XIONG You-hua~1,HUANG Bang-hai~3 (1.Zhongkai University of Agriculture and Engineering,Guangzhou,Guangdong 510225,2.Hechi University,Hechi,Guangxi 546300,3.Agricultural Technology Extension Center,Guangdong 510520) |
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| Abstract: | With a wide range of origin,ornamental Hedychium germplasms including cultivated species,varieties and new breeding materials are morphology difficult to identify.In this study,on the basis of thorough collecting of ornamental Hedychium germplasms,two pair of primer with total of eleven bands were selected from twelve primers to construct SRAP DNA fingerprint.The results showed that,through the SRAP fingerprint construction method,the polymorphic bands were clear,repeatedly,and could be used for the identif... |
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| Keywords: | Hedychium ornarmental germplasm SRAP fingerprint |
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