| 高灌蓝越橘多酚氧化酶的动力学研究 |
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| 引用本文： | 杨萌,B.Rovel,M..Metche.高灌蓝越橘多酚氧化酶的动力学研究[J].食品科学,2003,24(4):55-58. |
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| 作者姓名： | 杨萌 B.Rovel M..Metche |
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| 作者单位： | 1. 江南大学食品学院,无锡,214036
2. 法国国立洛林理工学院应用生物化学实验室 |
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| 摘 要： | 采用紫外区域内对底物的扫瞄法研究了高灌蓝越橘(Vaccynium corybosum L)多酚氧化酶的底物专一性，用紫外分光光度法和极谱测氧消耗法对酶的动力学进行了研究，测定了相应的米氏常数。用二类抑制剂研究抑制剂浓度对抑制剂效果的影响以及抑制剂与酶结合不同时间对抑制效果的影响，从而对高灌蓝越橘多酚氧化酶进行了抑制研究。
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| 关 键 词： | 高灌蓝越橘 多酚氧化酶 动力学 底物专一性 紫外分光光度法 极谱测氧消耗法 抑制剂 |
| 文章编号： | 1002-6630(2003)04-0055-04 |
Enzymatic Kinetics Study on Polyphenol Oxidase of Highbush Blueberry |
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| B.Rovel,M..Metche.Enzymatic Kinetics Study on Polyphenol Oxidase of Highbush Blueberry[J].Food Science,2003,24(4):55-58. |
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| Authors: | BRovel MMetche |
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| Affiliation: | YangMengetal. |
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| Abstract: | The substrate specificity of highbush blueberry polyphenol (Vaccyn iu m corybosum L.) oxidase has been studied by a substrate scanning method in spec trophotometric range. The enzymeatic kinetics has been investigated by using th e spectrophotometric method and poloragraphe method. Michaelis-constant(Km) has been assayed. The kinds of inhibitors and the effects of inhibitor concentrati o n and of combination time of inhibitor and enzyme on the inhibition of PPO hav e also been stadied. |
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| Keywords: | PPO substrate specificity kinetics of enzyme Michaelis-constant inhib itor |
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