| RP-HPLC法测定头孢噻肟钠的含量 |
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| 引用本文： | 陈向明,代现平,孙居锋.RP-HPLC法测定头孢噻肟钠的含量[J].中国药房,2010(36):3437-3438. |
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| 作者姓名： | 陈向明 代现平 孙居锋 |
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| 作者单位： | 滨州医学院药学院,烟台市264003 |
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| 摘 要： | 目的:建立以反相高效液相色谱法测定头孢噻肟钠含量的方法。方法:色谱柱为ultimate-C18,流动相为1.0 g.L-1的三乙量胺水为2溶0液μ(L用。磷结酸果调:p头H孢值噻至肟6.2钠)在-2.甲7醇×1(608-∶63～20.)0,2流0速7 g为.1L.0-1(mrL=.0m.i9n9-91 9,检)的测范波围长为内2线54性n关m,系柱良温好:25,检℃出,限流速为9为×11.00-9m gL..Lmi-1n-1,RS,进D=样0.18%。结论:所建立的方法分析速度快、灵敏度高,可用于头孢噻肟钠产品的含量测定。
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| 关 键 词： | 高效液相色谱法 紫外检测 头孢噻肟钠 |
Content Determination of Cefotaxime Sodium by RP-HPLC |
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| CHEN Xiang-ming,DAI Xian-ping,SUN Ju-feng.Content Determination of Cefotaxime Sodium by RP-HPLC[J].China Pharmacy,2010(36):3437-3438. |
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| Authors: | CHEN Xiang-ming DAI Xian-ping SUN Ju-feng |
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| Affiliation: | (School of Pharmacy,Binzhou Medical University,Yantai 264003,China) |
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| Abstract: | OBJECTIVE： To establish RPHPLC method for the content determination of cefotaxime sodium.METHODS： A ultimateC18 column was used with 1.0 g·L1 triethylamine solution（pH value adjusted to 6.2 with phosphoric acid）methanol（68 ∶ 32） as mobile phase at a flow rate of 1.0 mL·min1.The detection wavelength was set at 254 nm and column temperature was 25 ℃.The injection volume was 20 μL.RESULTS： The cefotaxime sodium showed good linear relationship within 2.7×106～0.020 7 g·L1（r=0.999 9） and the detection limit was 9×109 g·L1（RSD=0.18%）.CONCLUSION： The method is rapid,sensitive and suitable for the content determination of cefotaxime sodium. |
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| Keywords: | HPLC UV detection Cefotaxime sodium |
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