HPLC法同时测定抗敏颗粒中芍药苷、毛蕊异黄酮苷和阿魏酸的含量

目的:建立以高效液相色谱法同时测定抗敏颗粒中芍药苷、毛蕊异黄酮苷和阿魏酸含量的方法。方法:色谱柱为KromasiL C18(250mm×4.6mm,5μm),流动相为甲醇-乙腈-0.4%磷酸溶液(20∶10∶70),流速为1.0mL·min-1,检测波长为254nm,柱温为室温,进样量为10μL。结果:芍药苷、毛蕊异黄酮苷和阿魏酸的检测浓度分别在180～3600μg·mL-1(r=0.9992)、4.6～92.0μg·mL-1(r=0.9991)和8.0～160.0μg·mL-1(r=0.9994)范围内与各自峰面积积分值呈良好线性关系;平均回收率分别为98.3%(RSD=1.8%,n=9)、99.0%(RSD=2.0%,n=9)和100.5%(RSD=1.1%,n=9)。结论:本法操作简便、准确、灵敏、重现性好,可用于抗敏颗粒的质量控制。

Simultaneous Determination of Peoniflorin, Campanulin and Ferulic Acid in Kangmin Granules by HPLC

OBJECTIVE： To establish an HPLC method for simultaneous determination of peoniflorin,calycosin and ferulic acid in Kangmin granules.METHODS： The separation was performed on a Kromasil C18 column （250 mm×4.6 mm,5 μm）,and the mobile phase consisted of methanol-acetonitrile-0.4% phosphonic acid （20 ∶ 10 ∶ 70） with flow rate of 1.0 mL·min-1.The detection was performed at wavelength of 254 nm.Column temperature was room temperature and the injection volume was 10 μL.RESULTS： The linear ranges of peoniflorin,calycosin and ferulic acid were 180～3 600 μg·mL-1（r=0.999 2）,4.6～92.0 μg·mL-1（r=0.999 1） and 8.0～160.0 μg·mL-1（r=0.999 4）,respectively.The average recoveries were 98.3%（RSD=1.8%,n=9）,99.0% （RSD=2.0%,n=9） and 100.5%（RSD=1.1%,n=9）,respectively.CONCLUSION： The method is simple,accurate,sensitive and reproducible for the quality control of Kangmin granules.