| 骨肉瘤组织中RASSF1A基因启动子区甲基化状态观察及意义 |
| |
| 引用本文： | 章振华,桂斌捷,朱立新,李晓明,周健. 骨肉瘤组织中RASSF1A基因启动子区甲基化状态观察及意义[J]. 山东医药, 2012, 52(2): 24-26 |
| |
| 作者姓名： | 章振华 桂斌捷 朱立新 李晓明 周健 |
| |
| 作者单位： | 1. 安徽医科大学第一附属医院,合肥,230022
2. 香港中文大学 |
| |
| 基金项目： | 国家自然科学基金资助项目 |
| |
| 摘 要： | 目的 观察骨肉瘤组织中Ras相关区域家族1A(RASSF1A)基因启动子区域的甲基化状态,并探讨其临床意义.方法 应用甲基化特异性PCR(MSP)技术检测30例骨肉瘤组织及瘤旁组织中RASSF1A基因启动子区域的甲基化状态.结果 骨肉瘤组织中RASSF1A基因启动子区域发生甲基化14例(46.7％),瘤旁组织中4例(13.3％),两者比较,P＜0.05.RASSF1A基因启动子区甲基化与骨肉瘤患者的性别、年龄及血清碱性磷酸酶水平有关(P均＜0.05).结论 骨肉瘤组织中RASSF1A基因启动子区甲基化发生率高,其可能在骨肉瘤的发生发展中发挥重要作用.
|
| 关 键 词： | DNA甲基化 Ras相关区域家族1A基因 骨肿瘤 骨肉瘤 |
Observation of promoter methylation of RASSF1A gene in osteosarcoma tissue |
| |
| ZHANG Zhen-hua , GUI Bin-jie , ZHU Li-xin , LI Xiao-ming , ZHOU Jian. Observation of promoter methylation of RASSF1A gene in osteosarcoma tissue[J]. Shandong Medical Journal, 2012, 52(2): 24-26 |
| |
| Authors: | ZHANG Zhen-hua GUI Bin-jie ZHU Li-xin LI Xiao-ming ZHOU Jian |
| |
| Affiliation: | 1 First Affiliated Hospital of Anhui Medical University,Hefei 230022,P.R.China) |
| |
| Abstract: | Objective To observe the status of promoter methylation of Ras association domain family protein 1 A(RASSF1A)gene in osteosarcoma tissue,and explore the clinical significance.Method Methylation-specific PCR was adopted to investigate the promoter methylation of RASSF1A gene in 30 pairs of osteosarcoma and corresponding non-tumor tissues.Results The frequency of promoter hypermethylation of RASSF1A showed 46.7% and 13.3% in tumor and coresponding non-tumor tissues respectively,and there was significant diference between them(P<0.05).Promoter methylation of RASSF1A in osteosarcoma was related to the gender,age and Alkaline phosphatase(ALP).Conclusion RASSF1A methylation is a frequent event in osteosarcoma and probably participates in the progression of osteosarcoma. |
| |
| Keywords: | DNA methylation Ras association domain family protein 1 A gene osteosarcoma bone neoplasms |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
