靶向HIF-2 siRNA对人胰腺癌BXPC-3细胞增殖和凋亡的影响

目的观察靶向缺氧诱导因子2(HIF-2)的小干扰RNA(siRNA)对人胰腺癌BXPC-3细胞增殖和凋亡的影响。方法将BXPC-3细胞随机分为A、B、C组,A组转染HIF-2 siRNA、B组转染非特异性siRNA、C组不转染,分别予常氧、缺氧环境下培养;采用MTT法检测培养24、48、72、96 h时BXPC-3细胞的光密度(OD)值观察增殖情况,用流式细胞仪检测培养48 h时BXPC-3细胞凋亡率。结果在缺氧培养48 h时,A、B、C组细胞OD值分别为0.54±0.07、0.69±0.09、0.72±0.13,72 h时分别为0.55±0.11、0.88±0.07、0.88±0.05,96 h时分别为0.56±0.12、0.93±0.11、0.94±0.09;A组与B、C组比较,P均<0.05。在缺氧培养48 h时,A、B、C组细胞凋亡率分别为21.37%±0.17%、7.12%±0.03%、7.24%±0.07%;A组与B、C组比较,P均<0.05。在常氧状态下,各组细胞OD值及凋亡率无明显差别。结论缺氧状态下,靶向HIF-2 siRNA可抑制人胰腺癌BXPC-3细胞增殖,诱导其凋亡。

Effect of HIF-2 siRNA on proliferation and apoptosis in human pancreatic carcinomacell line BXPC-3

Objective To observe the effect of hypoxia-inducible factor 2(HIF-2)small interference RNA(siRNA) on the proliferation and apoptosis in human pancreatic carcinoma BXPC-3 cells.Methods BXPC-3 cells were divided into group A,B and C randomly.Cells in group A were transfected with HIF-2 siRNA,in group B with nonspecific siRNA and group C uninfected.Cells in each group were cultured with normoxic environment and hypoxic state.The cell proliferation was assessed by MTT assay at 24,48,72,96 h and the changes in cell apoptosis were evaluated by flow cytometry at 48 h.Results At the 48 h of hypoxic state,the OD value of group A,B and C were respectively 0.54±0.07,0.69±0.09,0.72±0.13,at the 72 h are respectively 0.55±0.11,0.88±0.07,0.88±0.05,at 96 h were respectively 0.56±0.12,0.93±0.11,0.94±0.09.Compared with group B and C,cell proliferation in group A was inhibited remarkably(all P<0.05).At the 48 h of hypoxic state culture,the apoptosis rate of group A,B and C were respectively 21.37%±0.17%,7.12%±0.03%,7.24%±0.07%.Compared with group B and C,the apoptosis in group A was increased significantly(all P<0.05).In normoxic environment,there was no significant difference between the OD value and apoptosis rates of each group.Conlusion In hypoxic environment,HIF-2 siRNA can inhibit cell proliferation,promote apoptosis in human pancreatic carcinoma cells.