| 带信号肽人VEGF165 cDNA克隆 |
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| 引用本文： | 何延政,谢毅.带信号肽人VEGF165 cDNA克隆[J].泸州医学院学报,2000,23(4):253-256. |
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| 作者姓名： | 何延政 谢毅 |
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| 作者单位： | [1]泸州医学院附属医院血管外科,泸州 [2]上海复旦大学遗传工程国家重点实验室,泸州 |
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| 基金项目： | 上海市教委资助课题 !( 95GB0 4 ) |
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| 摘 要： | 目的：克隆带信号肽人ＶＥＧＦ１６５ ｃＤＮＡ，以便进一步在真核系统表达人ＶＥＧＦ１６５蛋白或基因治疗。方法：设计引物；从人胎脑ｃＤＮＡ文加ＰＣＲ扩增目的基因；ＤＮＡ片段回收与酶切鉴定；与Ｍ１３ｍｐ１８重组；单链序列测定。结果：克隆片段与人ＶＥＧＦ１６５ｃＤＮＡ序列一致。结论：克隆ＤＮＡ片段为带信号肽人ＶＥＧＦ１６５ ｃＤＮＡ。
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| 关 键 词： | 带信号肽 VEGF165 cDNA 克隆 |
CLONING HUMAN VASCULAR ENDOTHELIAL GROWTH FACTOR(VEGF) cDNA WITH SIGNAL PEPTIDE CODE |
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| He Yanzheng,et al Research Division of Vascular Surgery,Luzhou Medical College Hospital.CLONING HUMAN VASCULAR ENDOTHELIAL GROWTH FACTOR(VEGF) cDNA WITH SIGNAL PEPTIDE CODE[J].Journal of Luzhou Medical College,2000,23(4):253-256. |
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| Authors: | He Yanzheng Research Division of Vascular Surgery Luzhou Medical College Hospital |
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| Abstract: | Objective:To clone human VEGF cDNA with signal peptide code,so that it may be expressed in eukaryotic system to produce human VEGF165 protein or used in gene therapy .Method:Primers was designed and synthesized .VEGF cDNA was amplified from human fetal brain cDNA library using PCR and the amplified fragment was inserted into M13mp18 and confirmed to be VEGF165cDNA with signal peptide code by restriction mapping and DNA sequencing .Result:The resulting sequence is identical to that of the VEGF165cDNA .Conclusion:the cloned cDNA is human vascular endothelial growth factor(VEGF)cDNA with signal peptide code. |
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| Keywords: | VEGF cDNA Cloning |
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