离子色谱测定唾液葡萄糖含量方法的建立及评估

目的建立用离子色谱测定唾液中葡萄糖浓度的方法。方法利用热变性法去除唾液中的蛋白质,以CarboPac PA20(3×30 mm)作为保护柱,CarboPac PA20(3×150 mm)作为分析柱进行离子色谱分析。以超纯水(A),250 mmol/L NaOH溶液(B),500 mmol/L NaAc(C)为淋洗液进行梯度洗脱,采用脉冲安培检测器检测。结果本方法在0.04~0.12 mg/L范围内具有较好的线性关系,线性相关系数R2=0.996 7;葡萄糖的检出限是0.002 mg/L;重复性测量相对标准偏差(RSD)的平均值为0.75%,加标回收率平均值为103.07%。结论本方法操作简便、灵敏度高、准确性好、结果稳定,可用于唾液中葡萄糖含量的测定。

Establishment,evaluation,and determination of saliva glucose concentration by ion chromatography

Objective To establish an analytical method for measuring the concentration of glucose in saliva by ion chromatography.Methods The proteins in saliva were removed by thermal denaturation method,CarboPac PA20(3×30 mm)was used as a protective column and CarboPac PA20(3×150 mm)was used as an analytical column for ion chromatography analysis.Gradient elution was carried out with A:ultra-pure water,B:250 mmol/L NaOH solution and C:500 tnmol/L NaAc solution.Pulsed ampere detector was used for detection.Results This method had a good linear relationship in the range of 0.04 to 0.12 mg/L,with a linear relation coefficient of 0.9967.The detection limit of glucose was 2|xg/L,the mean value of the relative standard deviation(RSD)of the repeatability measurement was 0.75%,and the average spike recovery was 103.07%.Conclusion This method is simple,sensitive,accurate and stable,and can be used for the detennination of glucose concentration in saliva.