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不同采收期连翘、金钟花果实中连翘酯苷和连翘苷的含量测定
引用本文:曲欢欢,李白雪,燕菲,孙文基. 不同采收期连翘、金钟花果实中连翘酯苷和连翘苷的含量测定[J]. 中国中医药信息杂志, 2008, 15(8): 47-48
作者姓名:曲欢欢  李白雪  燕菲  孙文基
作者单位:1. 西北大学陕西省生物医药重点实验室,陕西西安,710069
2. 成都中医药大学,四川成都,610075
3. 中国人民解放军第二军医大学药学院,上海,200433
摘    要:目的考察不同采收期连翘、金钟花果实中连翘酯苷和连翘苷的含量。方法采用HPLC-PDA法,以Diamonsil-18(4.6mm×250mm,5μm)为色谱柱,流动相为甲醇-水,梯度洗脱,流速为1mL/min,检测波长270nm。结果连翘果实中连翘酯苷的含量远高于金钟花,金钟花果实中不含连翘苷。结论不同采收期连翘、金钟花果实中连翘酯苷和连翘苷的含量有较大差别,金钟花不应混作连翘使用。

关 键 词:连翘  金钟花  连翘酯苷  连翘苷  含量测定

Determination of Forsythiaside and Forsythin from Fruits of Forsythia Suspensa and Forsythia Viridissima Lindl Collected in Different Periods
Affiliation:QU Huan-huan,LI Bai-xue,YAN Fei,et al(1.Biology and Medicine Key Laboratory of Shanxi Province, Northwest University, Xi'an 710069, China;2.Chengdu University of TCM, Chengdu 610075, China;3. School of Pharmacy, Second Military Medical University of PLA, Shanghai 200433, China)
Abstract:Objective To study the content of forsythiaside and forsythin from fruits of Forsythia suspensa and Forsythia viridissima Lindl collected in different periods. Methods Samples were dealt by HPLC-PDA with Diamonsil-C18 (4.6 mm × 250 mm, 5μm) column. The mobile phase consisted of methanol-water, gradient elution, the flow rate was 1.0 mL/min. The UV detection was set at 270 nm. Results The content of forsythiaside from fruits of Forsythia suspensa was much higher than that from fruits of Forsythia viridissima Lindl. And there was no forsythin from fruits of Forsythia viridissima Lindl. Conclusion There was much difference in content of forsythiaside and forsythin from the two kinds of fruits above. Forsythia viridissima Lindl should not be used as Forsythia suspensa.
Keywords:Forsythia suspensa  Forsythia viridissima Lindl  forsythiaside  forsythin  
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