山茱萸提取、纯化工艺考察

目的:优选山茱萸的提取工艺及大孔树脂纯化工艺。方法:以莫诺苷含量为指标,选择乙醇体积分数、料液比、提取时间、提取次数为考察因素,采用单因素试验和正交试验优选提取工艺。选取上样量、洗脱速度、洗脱剂浓度及用量为考察因素,采用单因素试验考察HPD-300型大孔吸附树脂纯化工艺。结果:山茱萸最佳提取工艺为加8倍量50%乙醇提取3次,每次3 h。纯化工艺为3 BV 30%乙醇以1.5 BV·h-1洗脱,洗脱率达94.9%。结论:优选的提取工艺稳定可行;HPD-300型大孔吸附树脂可较好地纯化山茱萸总苷。

Investigation of Extraction and Purification Technology of Cornus officinalis

Objective:To optimize extraction technology of Cornus officinalis,and investigate its purification technology by macroporous resin. Method: With the content of morroniside as index,the concentration of ethanol,solid-liquid ratio,extraction time and times were selected as factors,extraction technology of total glycosides was optimized by single factor test and orthogonal test.With sampling volume,elution speed,eluent concentration and dosage as factors,single factor test was used to investigate purification technology of C. officinialis with HPD-300 macroporous resin. Result: Optimum extraction technology was as following:extracted 3 times with 50% ethanol of 8 times amount,3 h each time.Purification technology was:eluted with 3 BV 30% ethanol at 1.5 BV·h^-1,elution rate of 94.9%. Conclusion: Optimized extracting technology was stable and feasible;It showed that total glycosides from C. officinalis could be effectively purify by HPD-300 macroporous resin.