| 双基因溶瘤腺病毒联合5-FU抑制肺癌细胞增殖的研究 |
| |
| 引用本文： | 王世兵,孟树林,武虎,马步云.双基因溶瘤腺病毒联合5-FU抑制肺癌细胞增殖的研究[J].浙江工程学院学报,2014(4):445-450. |
| |
| 作者姓名： | 王世兵 孟树林 武虎 马步云 |
| |
| 作者单位： | [1]浙江理工大学生命科学学院科研实验中心,杭州310018 [2]浙江理工大学新元医学与生物技术研究所,杭州310018 |
| |
| 基金项目： | 国家自然科学基金（51272236,51002139）; 浙江省自然科学基金（LY13H080005）; 浙江省公益性技术应用研究计划项目（2014C37101） |
| |
| 摘 要： | 研究携带TRAIL和Smac的双基因溶瘤腺病毒（ZD55-TRAIL-IETD-Smac）联合化疗药物5-氟尿嘧啶（5-FU）对肺癌细胞的体外杀伤作用。通过MTT法检测细胞抑制率,流式细胞术检测细胞凋亡率,Western Blot检测杀伤性基因表达与凋亡相关蛋白表达。结果显示ZD55-TRAIL-IETD-Smac与5-FU联合应用能有效地抑制肺癌细胞的增殖,并通过激活Caspase通路诱导肺癌细胞产生凋亡,表明,5-FU能够显著增强携带TRAIL和Smac的双基因溶瘤腺病毒对肺癌细胞的杀伤作用,为肺癌的临床应用提供了参考。
|
| 关 键 词： | ZD55-TRAIL-IETD-Smac 5-FU 肺癌 凋亡 |
Study on Inhibition of Lung Cancer Cell Proliferation by Combination of Dual-Gene Oncolytic Adenovirus with 5-FU |
| |
| WANG Shi-bing,MENG Shu-lin,WU Hu,MA Bu-yun.Study on Inhibition of Lung Cancer Cell Proliferation by Combination of Dual-Gene Oncolytic Adenovirus with 5-FU[J].Journal of Zhejiang Institute of Science and Technology,2014(4):445-450. |
| |
| Authors: | WANG Shi-bing MENG Shu-lin WU Hu MA Bu-yun |
| |
| Affiliation: | 1. Scientific Research Experiment Center, College of Life Science; 2. Xinyuan Institute of Medicine and Biotechnology, Zhejiang Sci-Tech University, Hangzhou 310018, China) |
| |
| Abstract: | The study explores the in-vitro lethal effects of dual-gene oncolytic adenovirus（ZD55-TRAIL-IETD-Smac）with TRAIL and Smac in combination of 5-FU on lung cancer cells.Cell inhibition ratio,apoptosis rate,destruction gene expression apoptosis-related protein expression were detected by MTT,flow cytometry and Western Blot respectively.The results show that combined application of ZD55-TRAIL-IETD-Smac and 5-FU can effectively inhibit the proliferation of lung cancer cells and induce apoptosis of lung cancer cells through activating Caspase passage.This indicates that 5-FU can significantly enhance lethal effects of dual-gene oncolytic adenovirus with TRAIL and Smac on lung cancer cells.This provides reference for clinical application of lung cancer. |
| |
| Keywords: | ZD55-TRAIL-IETD-Smac 5-FU lung cancer apoptosis |
| 本文献已被 维普 等数据库收录！ |
