Isolation and determination of p-hydroxybenzoylcholine in traditional Chinese medicine Semen sinapis Albae |
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| Authors: | Liu Lifang Liu Tao Li Genxi Wang Qiang Ng Tzibun |
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| Affiliation: | (1) Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, 210093 Nanjing, P. R. China;(2) Department of Pharmacognosy, China Pharmaceutical University, 210009 Nanjing, P. R. China;(3) Department of Biochemistry, The Chinese University of Hongkong, Shatin, Hongkong, P. R. China |
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| Abstract: | A special component is isolated from Semen sinapis Albae (white mustard seed), a traditional Chinese medicine. According to the physical and chemical investigation and spectroscopic identification, this component can be known as p-hydroxybenzoylcholine bisulfate, a choline base. This component in the drug is also determined by RP-HPLC. A reversed-phase C18 column is used to separate the p-hydroxybenzoylcholine with an eluent of methanol–0.05 mol/L monopotassium phosphate solution (30:70) (adjusted by phosphoric acid to pH 3.6) at the flow rate of 0.5 mL/min. Detection is carried out with a UV detector operated at 285 nm, and the column temperature is 25 °C. It reveals that there is 0.021% (w/w) of p-hydroxybenzoylcholine bisulfate in Semen sinapis Albae and 0.037% (w/w) in stir-baked Semen sinapis Albae. |
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| Keywords: | Semen sinapis Albae p-Hydroxybenzoylcholine bisulfate HPLC Determination |
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