36个马铃薯品种的SSR分析

为明确36个马铃薯品种在DNA分子水平上的遗传差异,利用SSR分子标记技术对其多态性进行了分析。试验从90对SSR引物中筛选出适宜于36个马铃薯品种基因组DNA扩增的引物10对,PCR扩增获得301个SSR条带,多态性条带百分率为71.1%。引物STACCA3和SSR111扩增的SSR指纹图谱差异明显,可作为36个马铃薯品种间鉴别的分子依据。36个马铃薯品种间的遗传距离(GD)变幅为0.33～0.85,平均为0.54,其中,大于GD平均值的品种有20个,占供试品种的55.56%。以GD值0.57为基准,将36个马铃薯品种划分成7类,从DNA分子水平揭示出各供试品种间的亲缘关系。

SSR Analysis on 36 Potato Varieties

To know the genetic differences in DNA level among 36 potato varieties,the polymorphism among the tested plants were analyzed based on SSR molecular marker.10 primer combinations,which suited the genome DNAs of 36 potato varieties,were selected from the 90 primer combinations.301 bands of SSR markers were amplified,the percentage of polymorphism bands reached 71.1%.The differences in SSR fingerprints amplified with primers STACCA3 and SSR111 were significant.It can be used as molecular evidence to distinguish 36 potato varieties.The genetic distance(GD)of 36 tested plants ranged from 0.33 to 0.85,and the average of genetic distance was 0.54.There were 20 varieties,GD of which was greater than the average GD,accounting for 55.56% of the varieties tested.Using the GD value of 0.57 as threshold,36 tested plants could be clustered into 7 groups,the genetic relationships among tested varieties were revealed at DNA molecular level.