| 苏云金杆菌抗虫基因cryIAc转化辣椒的研究 |
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| 引用本文： | 袁静,舒庆尧,刘中来.苏云金杆菌抗虫基因cryIAc转化辣椒的研究[J].植物科学学报,2004,22(3):201-204. |
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| 作者姓名： | 袁静 舒庆尧 刘中来 |
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| 作者单位： | 1. 华中师范大学分子遗传学实验室, 武汉 430079;2. 浙江大学原子核农业科学研究所, 杭州 310029 |
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| 摘 要： | 通过农杆菌介导法将杀虫结晶蛋白基因cryIAc导入到辣椒子叶外植体中,经卡那霉素筛选、组织化学法检测GUS活性以及PCR、Southern杂交分析证实cryIAc基因已整合进辣椒核基因组中。
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| 关 键 词： | 辣椒 Bt杀虫基因 农杆菌介导法 转基因植株 |
Study on Transformation of Insecticidal Bacillus thuringiensis cryIAc Gene in Pepper |
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| YUAN Jing,SHU Qing-Yao,LIU Zhong-Lai.Study on Transformation of Insecticidal Bacillus thuringiensis cryIAc Gene in Pepper[J].Plant Science Journal,2004,22(3):201-204. |
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| Authors: | YUAN Jing SHU Qing-Yao LIU Zhong-Lai |
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| Affiliation: | 1. Molecular Biology Laboratory, Central China Normal University, Wuhan 430079, China;2. Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310029, China |
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| Abstract: | The insecticidal Bacillus thuringiensis(Bt)cryIAc gene was used to transform the cotyledon explants of pepper via Agrobacterium transformation.Through stringent selection of kanamycin,analysis of the expression of GUS in the plants by histochemical method,molecular biological assays with PCR and Southern dot hybridization,it is confirmed that the cryIAc gene was integrated into the genome of pepper. |
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| Keywords: | Pepper Bt insecticidal gene Agrobacterium-mediated method Transgenic plant |
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