IL—2,IL—4基因转染后肿瘤细胞表面ICAM—1分子的表达及其作用

为了进一步研究细胞因子基因转染后肿瘤细胞体内致瘤原性、免疫原性变化的分子机制，将ＩＬ－２、ＩＬ－４基因转染入Ｂ１６黑色素瘤细胞，观察了其体内接种后致瘤原性变化，通过ＦＡＣＳ法检测了其细胞表面粘附分子－１（ＩＣＡＭ－１）的表达水平，分析了其细胞表面ＩＣＡＭ－１表达水平以及它们与肿瘤细胞对ＬＡＫ、ＣＴＬ等免疫效应细胞杀伤敏感性变化的关系。结果表明，ＩＬ－２、ＩＬ－４基因转染后Ｂ１６细胞体内致瘤原性明显

INCREASED EXPRESSION OF ICAM-1 OF B16 MELANOMA CELLS TRANSFECTED WITH IL-2 GENE, IL-4 GENE AND ITS SIGNIFICANCE

In order to further elucidate the molecular mechanisms which involve in changes of the tumorigenicity of cytokine gene-transfected tumor cells, we transfected IL-2 gene,IL-4 gene into B16 melanoma cells, and then observed their tumorigenicity in vivo. The expression level of ICAM-1 were also detected by FACS method. Sensitivities of the cytokine gene transfected B16 cells to LAK,CTL cytotoxicity were compared with that of the wild-type B16 cells. The results demonstrated that both IL-2 gene and IL-4 gene-transfected B16 cells have more increased ICAM- expression than the wild-type B16 cells. These cytokine gene modified B16 cells become more sensitive to LAK or CTL cytotoxicity, and that can be abolished by anti-ICAM-1 monoclonal antibody. The present result suggested that the decreased tumorigenicity of the IL-2 gene,IL-4 gene transfected B16 melanoma cells can be attribute to not only their increased antitumor immunity of the host, but also to the increased sensitivitiy to effector cells by increased ICAM-1 expression.