磷酸化STAT5、FOXP3在急性白血病患者骨髓中的表达及意义

目的观察急性白血病患者骨髓中单个核细胞磷酸化STAT5及FOXP3的表达水平及其临床意义。方法采用蛋白磷酸化流式细胞术分别检测45例急性白血病患者（AL）急性髓系白血病（AML）29例,急性淋巴细胞白血病（ALL）16例]及12名对照组骨髓单个核细胞磷酸化STAT5（P-STAT5）及FOXP3阳性细胞的百分率。结果 AL患者组骨髓单个核细胞PSTAT5表达（2.35±0.82）%较对照组（0.58±0.16）%增高,差异具有统计学意义（P〈0.05）;AL患者组FOXP3（3.98±1.17）%高于对照组（0.96±0.45）,差异具有统计学意义（P〈0.05）;AML组P-STAT5及FOXP3水平（2.51±0.91）%,（3.72±1.12）%和ALL组（2.27±0.78）%,（4.03±1.18）%比较无显著差异（P〉0.05）。AL患者P-STAT5及FOXP3表达水平显著正相关（r=0.81,P〈0.05）。结论磷酸化STAT5及Foxp3可能共同参与了急性白血病的发生过程,同时检测可以提供有临床意义的实验依据。

Phosphorylation STAT5, FOXP3 expression and significance in bone marrow of patients with acute leukemia

Objective To observe the expression and its clinical significance of P-STAT5 and FOXP3 in bone marrow mononuclear cells of patients with acute leukemia. Methods The percentage of P-STAT5 and FOXP3 positive cells in the bone marrow mononuclear cells were tested in 45 cases of patients with acute leukemia（AL）, including 29 cases of acute myeloid leukemia（AML） and 16 cases of acute lymphoblastic leukemia（ALL）, and 12 controls by using phospho-flow cytometry. Results The percentage of P-STAT5 in bone marrow mononuclear cells of AL patients group（2.35±0.82） was greatly higher than that of control group（0.58±0.16）, the difference had statistical significance（P〈0.05）. The percentage of FOXP3 in patients with AL（3.98±1.17） was higher than that of control group（0.96±0.45）, the difference had statistical significance（P〈0.05）. The percentage of PSTAT5 and FOXP3 had no significant difference between AML group（2.51±0.91,3.72±1.12） and ALL group（2.27±0.78,4.03±1.18）（P〈0.05）. The expression level of P-STAT5 and FOXP3 in AL patients had significantly positive correlation（r=0.81, P〈0.05）.Conclusion P-STAT5 and FOXP3 may be involved in the occurrence of acute leukemia, detection of P-STAT5 and FOXP3 at the same time can provide experimental basis with clinical significance.