MGMT基因多态性与食管癌易感性关系

目的 探讨山西省长治地区食管癌患者O6-甲基鸟嘌呤DNA甲基转移酶(MGMT)基因多态性与食管癌易感性的关系,及其与环境因素在食管癌发生中的交互作用。方法 采用1:1配对的病例对照研究,对201对研究对象进行食管癌相关危险因素的问卷调查,应用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术对MGMT多态性基因型进行检测。结果 携带MGMT基因杂合型和突变型者发生食管癌的风险增高,χ2=9.80,P<0.01,OR值分别为1.33(95%CI=1.01~2.07)和1.47(95%CI=1.03~2.54);MGMT易感基因型与腌制食品对食管癌的发生存在交互作用(S=1.77,API=0.25),而与吸烟、饮酒、精神刺激、食管癌家族史之间没有交互作用。结论 MGMT突变基因型是长治地区食管癌高发的易感基因型,且与腌制食品存在协同作用。

Relationship between MGMT gene polymorphism and susceptibility of esophageal cancer

Objective To explore the relationship of polymorphism of O⁶-methylguanine-DNA-methyltransferase (MGMT)and the susceptibility of esophageal cancer(EC),and the interaction with environmental factors-gene in Changzhi area.Methods The 1:1 matching case-control study included 201 EC cases and 201 controls.MGMT polymorphisms were determined with polymerase chain2restriction on fragment length polymorphism(PCR2RFL P).Results The risk of EC significantly increased in individuals with MGMT mutation genotype and heterozygote compared to those with wild genotype (χ²=9.80,P<0.01).ORs(95%CI)were 1.33(1.01～2.07)and 1.47(1.03～2.54),respectively.There was interaction between MGMT mutation genotype and pickled food(S=1.77,A PI=0.25).Conclusion A genetic polymorphism in the MGMT gene may be associated with esophageal cancer in Changzhi area,and there is synergistic action between MGMT predisposing genotype and pickled food.