| 酪蛋白磷酸肽的制备及性质 |
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| 引用本文： | 杨曜中 欧伶. 酪蛋白磷酸肽的制备及性质[J]. 华东理工大学学报(自然科学版), 1999, 25(6): 574-577 |
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| 作者姓名： | 杨曜中 欧伶 |
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| 作者单位： | 华东理工大学生物化学研究所!上海200237 |
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| 摘 要： | 用不同的蛋白水解酶水解牛乳酪蛋白，通过钙－乙醇沉淀法和离子交换法从水解产物中提取酪蛋白磷酸肽（ＣＰＰｓ），并对ＣＰＰｓ进行体外化学分析及体内生物学功能鉴定，结果表明：用双酶法（胰蛋白酶和Ａｌｃａｌａｓｅ）制得的ＣＰＰｓ３（平均相对分子质量为４ ０６６，摩尔Ｎ／Ｐ为５．４８）在体外的持钙能力最强，连续喂食该样品的小鼠对钙的吸收明显提高（吸收率达５６％ ）。
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| 关 键 词： | 酪蛋白磷酸肽 胰蛋白酶 Alcalase 制备 |
Preparation and Properties of Casein Phosphopeptides |
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| YANG Yao zhong ,QU Ling,GE Yi qun,SONG Yu wen. Preparation and Properties of Casein Phosphopeptides[J]. Journal of East China University of Science and Technology, 1999, 25(6): 574-577 |
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| Authors: | YANG Yao zhong QU Ling GE Yi qun SONG Yu wen |
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| Affiliation: | YANG Yao zhong *,QU Ling,GE Yi qun,SONG Yu wen |
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| Abstract: | Various casein phosphopeptides (CPPs) were extracted by Ca 2+ /ethanol precipitation and ion exchange chromatography through hydrolyzing milk casein enzymaticlly, and then were determined in vitro and in vivo. The results showed that CPPs3 (MW 4 066, N/P 5.48) derived from bienzymatic hydrolysate (trypsin and alcalase) is the best one in calcium binding capacity in vitro and in improving Ca 2+ absorption in mice (Ca 2+ absorption rate is 56%). These two data were superior to the CPPs derived from single enzymatic hydrolysate and other CPPs products. |
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| Keywords: | casein phospopeptides trypsin alcalase preparation property |
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