Vitrification,not cryoprotectant exposure,alters the expression of developmentally important genes in in vitro produced porcine blastocysts |
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| Affiliation: | 1. Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camden, NSW 2570, Australia;2. Westmead Fertility Centre, Westmead Hospital, Westmead, NSW 2145, Australia;1. Departamento de Ciencias de la Salud, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, 09340 DF, Mexico;2. Doctorado en Ciencias Biológicas y de la Salud, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, 09340 DF, Mexico;3. Departamento de Biología de la Reproducción, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, 09340 DF, Mexico;4. Departamento de Medicina y Cirugía Animal, Universidad de Murcia, Espinardo 30100, Spain;1. IVI Valencia, IVI-RMA Global, Plaza Policía Local, 3, 46015, Valencia, Spain;2. Embryotools, Parc Científic de Barcelona, Av. Doctor Marañon, 8, 08028, Barcelona, Spain;1. Yunnan Provincial Engineering Laboratory of Animal Genetic Resource Conservation and Germplasm Enhancement, Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan 650224, People''s Republic of China;2. College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan 650201, People''s Republic of China;1. Departamento de Ciencias de la Salud, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, 09340 DF, México;2. Departamento de Biología de la Reproducción, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, 09340 DF, México;3. Maestría en Biología de la Reproducción Animal, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa Av. San Rafael Atlixco 186, 09340 DF, México |
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| Abstract: | The vitrification of embryos is common practice in advanced livestock breeding programs and in human fertility clinics. Recent studies have revealed that vitrification results in aberrant expression of a number of stress related genes. However, few studies have examined the effect that vitrification has on developmentally important genes, and none have been conducted in porcine embryos. The aim of this study was to determine the effects that different vitrification procedures and cryoprotectant combinations have on the expression of imprinted genes in in vitro produced (IVP) porcine blastocysts. The transcript levels of insulin-like growth factor 2 (IGF2) were lower in all groups of vitrified blastocysts compared to that in non-vitrified control blastocysts (P < 0.05). Expression levels of IGF2 and IGF2 receptor (IGF2R) in blastocysts that had been exposed to cryoprotectants without being vitrified were similar to that in non-vitrified control blastocysts (P > 0.05). Furthermore, blastocysts vitrified using ethylene glycol and propanediol combined, and those vitrified in a closed device, had IGF2R transcript levels similar to that in non-vitrified control blastocysts (P > 0.05). In conclusion, vitrification, but not exposure to cryoprotectants, caused aberrant expression of the imprinted genes IGF2 and IGF2R. Vitrification protocols that incorporated propanediol or a closed device were found to be least disruptive of gene expression in IVP porcine blastocysts. |
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| Keywords: | Cryopreservation IGF2 IGF2R Imprinted gene Porcine blastocyst Vitrification |
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