尿脱落细胞6号染色体长臂的微卫星改变诊断膀胱肿瘤

目的：探讨尿脱落细胞6q的微卫星改变(MA)在膀胱肿瘤早期诊断中的应用价值，并研究6q的杂合性缺失(LOH)与膀胱肿瘤的关系．方法：应用6q21区域附近D6S404，D6S434微卫星标志，以PCR—SSLP—银染法对31例膀胱肿瘤的尿脱落细胞与肿瘤组织进行微卫星分析．结果：64．5％的肿瘤组织和58．1％的尿脱落细胞发生MA;10例非膀胱肿瘤对照未出现MA；检出率与肿瘤分期，分级无相关性；肿瘤组织D6S404 LOH发生率35．5％，D6S434 LOH发生率22．6％．结论：尿脱落细胞微卫星分析有早期诊断意义；6q21区域附近可能存在与膀胱肿瘤相关的肿瘤抑制基因．

Investigation on microsatellite alteration at chromosome 6q of urine sediment in bladder tumor

AIM: To investigate the microsatellite alteration (MA) at chromosome 6q in the early diagnosis of bladder tumor and to study whether the loss of heterozygosity (LOH) at 6q is related to the development of bladder tumor. METHODS: D6S404 and D6434 microsatellite markers near 6q21 were tested by PCR SSLP stain method in both the urine sediment DNA and tumor DNA from 31 cases of bladder tumor. RESULTS: Among the 31 cases of bladder tumor, 64.5% of the tumor tissue DNA and 58 1% of the urine sediments DNA showed MA, while the 10 non bladder tumor cases had no changes at either of the two loci. The result was not associated with the grade or stage of the tumor. LOH was detected in tumor tissues on site for D6S404 (35 5%) and D6S434 (22 6%). CONCLUSION: Microsatellite analysis of urine sediments is of significance in the early diagnosis of bladder tumor and there may be some tumor suppressor gene relevant to the development of bladder tumor near 6q21.