鸡蛋中磺胺类残留物提取方法

为获得鸡蛋中磺胺类药物残留检测的提取方法，分别在鸡蛋中添加高、中、低浓度的磺胺类药物，根据GB/T 20759-2006《畜禽肉中十六种磺胺类药物残留量的测定：液相色谱-串联质谱法》的方法以及自行设计方法对样品进行前处理，用液相色谱-串联质谱检测。结果表明：添加量在10～200ng/g范围内，鸡蛋中回收率为20.8%～69.29%，回收率未能达到标准；GB/T 20759-2006在提取、浓缩、除脂、标准曲线绘制方面对于禽蛋类食品不适用，需根据禽蛋类的特殊性制定专门的检测标准，而自行设计方法所得回收率为77.92%～95.26%，可以满足检测的要求。

A Comparative Study on LC-MS/MS Determination of Sulfonamide Residues in Hen,s Eggs Based on Different Sample Pretreatments

Two different sample pretreatment methods,namely the national standard method GB/T 20759-2006 and a method developed in our laboratory were compared for their application in LC-MS/MS determination of sulfonamide residues in hen,s eggs.The average recoveries of the national standard method across the spike levels of 10,100 ng/g and 200 ng/g ranged from 20.8%-67.7%,which could not meet the requirements.Moreover,the method was not suitable for use in the extraction,concentration,defatting,and standard curve preparation of egg foods.Therefore,the determination of sulfonamide residues in eggs requires a new specialized method due to their special characteristics.The average recoveries of the self-developed method were between 77.92% and 95.26% and could meet the requirements for the determination of sulfonamide residues.